Author(s): Naves, Lara Marques ; Marques, Stefanne Madalena ; Mourao, Aline Andrade ; Fajemiroye, James Oluwagbamigbe ; Xavier, Carlos Henrique ; Castro, Carlos Henrique de ; Silva Rebelo, Ana Cristina ; Rosa, Daniel Alves ; Gomes, Rodrigo Mello ; Colombari, Eduardo [UNESP] ; Pedrino, Gustavo Rodrigues
Date: 2018
Persistent ID: http://hdl.handle.net/11449/164462
Origin: Oasisbr
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Made available in DSpace on 2018-11-26T17:54:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-26
Made available in DSpace on 2018-11-26T17:54:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-26
Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
The infusion of hypertonic saline solution (HSS) is known to be beneficial to the treatment of hypovolemic hemorrhage (HH). The central mechanism of HSS-induced cardiovascular and autonomic recovery of animals subjected to HH remains unclear. Hence, the present study evaluated the involvement of median preoptic nucleus (MnPO) and medullary noradrenergic neurons (A1 and A2) in HSS-induced cardiovascular and sympathetic responses in hemorrhagic rats. The wistar rats were subjected to specific lesion of noradrenergic neurons through the nanoinjections of anti-D beta H-saporin into caudal ventrolateral medulla (A1 neurons) and nucleus of the solitary tract (A2 neurons). After recovery, mean arterial pressure (MAP) and renal sympathetic nervous activity were recorded. The HH was performed through blood withdrawal until a MAP of 60 mmHg was attained. In sham rats, HSS infusion (3M NaCl) reestablished MAP without change in HH-induced sympathoinhibition. The muscimol (agonist of GABA(A) receptor) was nanoinjected in MnPO during HH and MnPO inhibition abolished the recovery of MAP and HSS-induced sympathoinhibition. Simultaneous lesions of A1 and A2 abolished MAP restoration and sympathoinhibition after HSS infusion. These results suggest that the recovery of MAP and HSS-induced sympathoinhibition in hemorrhaged rats depend on intact neural projections from A1 and A2 to MnPO.
Univ Fed Goias, Inst Ciencias Biol, Dept Ciencias Fisiol, Estr Campus S-N, BR-74690900 Goiania, Go, Brazil
Univ Fed Goias, Fac Farm, Goiania, Go, Brazil
Univ Fed Goias, Inst Ciencias Biol, Dept Morfol, Estr Campus S-N, BR-74690900 Goiania, Go, Brazil
Univ Estadual Paulista, Dept Fisiol & Patol, Fac Odontol, Araraquara, SP, Brazil
Univ Estadual Paulista, Dept Fisiol & Patol, Fac Odontol, Araraquara, SP, Brazil
Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2012/0055431086
Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG): 2009/10267000352
CNPq: 477832/2010-5
CNPq: 483411/2012-4
CNPq: 447496/2014-0
