Author(s): Mesquita, F. S. ; Ramos, R. S. ; Pugliesi, G. ; Andrade, S. C. S. ; Van Hoeck, V. ; Langbeen, A. ; Oliveira, M. L. ; Gonella-Diaza, A. M. ; Gasparin, G. ; Fukumasu, H. ; Pulz, L. H. ; Membrive, C. M. [UNESP] ; Coutinho, L. L. ; Binelli, M.
Date: 2018
Persistent ID: http://hdl.handle.net/11449/164692
Origin: Oasisbr
Subject(s): endometrium; fertility; genomics; gonadal steroids; ruminants (cows, sheep, llama, camel)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
This study aimed to characterize the endometrial transcriptome and functional pathways overrepresented in the endometrium of cows treated to ovulate larger (>= 13 mm) versus smaller (<= 12 mm) follicles. Nelore cows were presynchronized prior to receiving cloprostenol (large follicle [LF] group) or not (small follicle [SF] group), along with a progesterone (P4) device on Day (D) - 10. Devices were withdrawn and cloprostenol administered 42-60 h (LF) or 30-36 h (SF) before GnRH agonist treatment (D0). Tissues were collected on D4 (experiment [Exp.] 1; n = 24) or D7 (Exp. 2; n = 60). Endometrial transcriptome was obtained by RNA-Seq, whereas proliferation and apoptosis were assessed by immunohistochemistry. Overall, LF cows developed larger follicles and corpora lutea, and produced greater amounts of estradiol (D - 1, Exp. 1, SF: 0.7 +/- 0.2; LF: 2.4 +/- 0.2 pg/ml; D - 1, Exp. 2, SF: 0.5 +/- 0.1; LF: 2.3 +/- 0.6 pg/ml) and P4 (D4, Exp. 1, SF: 0.8 +/- 0.1; LF: 1.4 +/- 0.2 ng/ml; D7, Exp. 2, SF: 2.5 +/- 0.4; LF: 3.7 +/- 0.4 ng/ml). Functional enrichment indicated that biosynthetic and metabolic processes were enriched in LF endometrium, whereas SF endometrium transcriptome was biased toward cell proliferation. Data also suggested reorganization of the extracellular matrix toward a proliferation-permissive phenotype in SF endometrium. LF endometrium showed an earlier onset of proliferative activity, whereas SF endometrium expressed a delayed increase in glandular epithelium proliferation. In conclusion, the periovulatory endocrine milieu regulates bovine endometrial transcriptome and seems to determine the transition from a proliferation-permissive to a biosynthetic and metabolically active endometrial phenotype, which may be associated with the preparation of an optimally receptive uterine environment.
Univ Fed Pampa, Curso Med Vet, Uruguaiana, RS, Brazil
Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Anim Reprod, BR-13635900 Sao Paulo, Brazil
Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Zootecnia, BR-13635900 Sao Paulo, Brazil
Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, BR-13635900 Sao Paulo, Brazil
Univ Estadual Paulista, Sao Paulo, Brazil
Univ Antwerp, Fac Pharmaceut Biomed & Vet Sci, Antwerp, Belgium
Univ Estadual Paulista, Sao Paulo, Brazil
