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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao de Amparo a Pesquisa do Estado do Piaui (FAPEPI)
Casearia sylvestris Swartz is a medicinal plant widely distributed in Brazil. It has anti-inflammatory, antiulcer and antitumor activities and is popularly used to treat snakebites, wounds, diarrhea, flu and chest colds. Its leaves are rich in oxygenated tricyclic cis-clerodane diterpenes, particulary casearins. Herein, we evaluated the antioxidant activities of a fraction with casearins (FC) isolated from C. sylvestris and histological changes on the central nervous system and livers of Mus musculus mice. Firstly, in vitro studies (0.9, 1.8, 3.6, 5.4 and 7.2 mu g/mL) revealed EC50 values of 3.7, 6.4 and 0.16 mu g/mL for nitrite, hydroxyl radical and TBARS levels, respectively. Secondly, FC (2.5, 5, 10 and 25 mg/kg/day) was intraperitoneally administered to Swiss mice for 7 consecutive days. Nitrite levels in the hippocampus (26.2, 27.3, 30.2 and 26.6 mu M) and striatum (26.3, 25.4, 34.3 and 27.5 mu M) increased in all treated animals (P < 0.05). Lower doses dropped reduced glutathione, catalase and TBARS levels in the hippocampus and striatum. With the exception of this reduction in TBARS formation, FC displayed only in vitro antioxidant activity. Animals exhibited histological alterations suggestive of neurotoxicity and hepatotoxicity, indicating the need for precaution regarding the consumption of medicinal formulations based on Casearia sylvestris.
Univ Fed Piaui, Programa Posgrad Ciencias Farmaceut, BR-64049550 Teresina, PI, Brazil
Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil
Univ Estadual Ceara, Fac Med Vet, BR-60000000 Fortaleza, Ceara, Brazil
Univ Fed Piaui, Dept Ciencias Biol, BR-64607670 Picos, PI, Brazil
Univ Estadual Paulista, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil
Univ Fed Ceara, Dept Fisiol & Farmacol, BR-60430270 Fortaleza, Ceara, Brazil
Univ Fed Piaui, Dept Biofis & Fisiol, BR-64049550 Teresina, PI, Brazil
Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil
Univ Estadual Paulista, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil
CNPq: 484286/2011-0
Fundacao de Amparo a Pesquisa do Estado do Piaui (FAPEPI): 034/2012-PPP
