Author(s): Del Collado, Maite [UNESP] ; Saraiva, Naiara Z. ; Lopes, Flavia L. [UNESP] ; Gaspar, Roberta C. [UNESP] ; Padilha, Luciana C. [UNESP] ; Costa, Roberta R. ; Rossi, Guilherme F. [UNESP] ; Vantini, Roberta [UNESP] ; Garcia, Joaquim M. [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/173483
Origin: Oasisbr
Subject(s): in vitro production (IVP); lipid droplet; mitochondria; reproduction
Made available in DSpace on 2018-12-11T17:05:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01
Proper oocyte maturation is crucial for subsequent embryo development; however, oocyte mitochondrial and lipid-droplet behaviour are still poorly understood. Although excessive lipid accumulation during in vitro production (IVP) of bovine embryos has been linked with impaired cryotolerance, lipid oxidation is essential for adequate energy supply. Fetal bovine serum (FBS) and bovine serum albumin (BSA) are supplements used during IVP, containing high and low lipid content, respectively. This study aimed to understand how these supplements influence oocyte mitochondrial and lipid behaviour during in vitro maturation (IVM) in comparison to in vivo maturation, as well as their influence on development rates and embryo lipid accumulation during IVP. We demonstrate that only in vivo-matured oocytes maintained correlation between lipid content and active mitochondria. IVM media containing FBS increased total lipid content 18-fold and resulted in higher lipid accumulation in oocytes when compared with media with BSA. IVM using a lower FBS concentration combined with BSA resulted in satisfactory maturation and embryo development and also reduced lipid accumulation in blastocysts. In conclusion, IVM causes changes in mitochondrial and lipid dynamics, which may have negative effects on oocyte development rates and embryo lipid accumulation. Moreover, decreasing FBS concentrations during IVM may reduce embryo lipid accumulation without affecting production rates.
Departamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos USP, Avenida Duque de Caxias Norte, 225
Departamento de Medicina Veterinária Preventiva e Reproduçao Animal Faculdade de Ciencias Agrárias e Veterinárias UNESP, Via de Acesso Prof. Paulo D. Castellane SN
Empresa Brasileira de Pesquisa Agropecuária Embrapa Amazônia Oriental, Travessa Dr. Enéas Pinheiro s/n
Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba UNESP, Rua Clóvis Pestana, 793
Departamento de Biologia Celular e Molecular e de Bioagentes Patogênicos Faculdade de Medicina de Ribeirão Preto USP, Avenida Bandeirantes, 3900
Departamento de Medicina Veterinária Preventiva e Reproduçao Animal Faculdade de Ciencias Agrárias e Veterinárias UNESP, Via de Acesso Prof. Paulo D. Castellane SN
Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba UNESP, Rua Clóvis Pestana, 793
