Author(s): Gomes, Laise de Azevedo ; Moraes, Pablo Henrique Gonçalves ; do Nascimento, Luciana de Cássia Silva ; O'Dwyer, Lucia Helena [UNESP] ; Nunes, Márcio Roberto Teixeira ; Rossi, Adriana dos Reis Ponce ; Aguiar, Délia Cristina Figueira ; Gonçalves, Evonnildo Costa
Date: 2018
Persistent ID: http://hdl.handle.net/11449/173670
Origin: Oasisbr
Subject(s): Canine hepatozoonosis; Hepatozoon americanum; Hepatozoon canis; Molecular epidemiology; Nested PCR
Made available in DSpace on 2018-12-11T17:07:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01
This study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Belém, Pará, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp. using a new nested PCR assay. Positive samples were subjected to genetic characterization based on amplification and sequencing of approximately 670 bp of the Hepatozoon spp. 18S rRNA. Of the positive dogs, four shared the haplotype Belém 01, one dog presented the haplotype Belém 02 and two dogs shared the haplotype Belém 03. A Bayesian inference indicates that haplotypes Belém 01 and Belém 02 are phylogenetically related to H. canis, while Belém 03 is related to H. americanum. Overall, based on the first molecular evidence of H. americanum in Brazilian domestic dogs, the proposed protocol may improve the epidemiological investigation of canine hepatozoonosis.
Laboratório de Tecnologia Biomolecular Instituto de Ciências Biológicas Universidade Federal do Pará—UFPA, Belém
Instituto de Biociências UNESP—Universidade Estadual Paulista Campus de Botucatu Departamento de Parasitologia, Distrito de Ribeirão Junior
Centro de Inovações Tecnológicas Instituto Evandro Chagas, Levilândia
Instituto de Biociências UNESP—Universidade Estadual Paulista Campus de Botucatu Departamento de Parasitologia, Distrito de Ribeirão Junior
