Author(s): Chies, Agnaldo Bruno ; de Oliveira, Priscilla Bianca ; Rossignoli, Patrícia de Souza [UNESP] ; Baptista, Rafaela de Fátima Ferreira ; de Lábio, Roger William ; Payão, Spencer Luiz Marques
Date: 2018
Persistent ID: http://hdl.handle.net/11449/173973
Origin: Oasisbr
Subject(s): Angiotensin II; Endothelin-1; Exercise; Nitric oxide; Prostanoids; Vein
Made available in DSpace on 2018-12-11T17:08:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.
Laboratory of Pharmacology Marília Medical School
Department of Physiotherapy and Occupational Therapy São Paulo State University (UNESP), Marília
Faculty Esefap of Tupã
Faculty of Alta Paulista (FAP), Tupã
Laboratory of Genetic Marília Medical School, São Paulo
Department of Physiotherapy and Occupational Therapy São Paulo State University (UNESP), Marília
