Autor(es): Lopes, Fernando Aguilar ; Rezende, Jania de ; Silva, Danielly Beraldo dos Santos [UNESP] ; Alves, Fernanda de Cássia Gonçalves ; Oliveira, Carina Elisei de ; Costa, Izaías Pereira da
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/175513
Origem: Oasisbr
Assunto(s): Baggio‐Yoshinari syndrome; Borrelia burgdorferi; Brazil; flgE; Lyme disease
Made available in DSpace on 2018-12-11T17:16:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-11-01
We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme‐like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar (flgE). The results showed positive serology and for the first time, the presence of Borrelia burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of Borrelia burgdorferi flgE gene. By neighbor‐joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of Borrelia burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil.
Universidade Federal de Mato Grosso do Sul (UFMS) Faculdade de Medicina (Famed) Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste
Universidade Federal de Mato Grosso do Sul (UFMS) Hospital Universitário Maria Aparecida Pedrossian (Humap)
Universidade Católica Dom Bosco (UCDB) Programa de Pós-Graduação em Biotecnologia
Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias (FCAV) Programa de Pós-Graduação em Genética e Melhoramento Animal
Universidade Católica Dom Bosco (UCDB) Programa Institucional de Bolsas de Iniciação Científica (PIBIC)
Universidade Estadual Paulista (Unesp) Faculdade de Ciências Agrárias e Veterinárias (FCAV) Programa de Pós-Graduação em Genética e Melhoramento Animal
