Author(s): Sylvestre, Tatiane Fernanda [UNESP] ; Cavalcante, Ricardo de Souza [UNESP] ; Da Silva, Julhiany de Fátima [UNESP] ; Miranda Paniago, Anamaria Mello ; Weber, Simone Schneider ; Pauletti, Bianca Alves ; Carvalho, Lídia Raquel de [UNESP] ; Dos Santos, Lucilene Delazari [UNESP] ; Mendes, Rinaldo Poncio [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/176793
Origin: Oasisbr
Made available in DSpace on 2018-12-11T17:22:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01
The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings.
Universidade Estadual Paulista (UNESP) Faculdade de Medicina de Botucatu
Universidade Federal de Mato Grosso do Sul (UFMS) Faculdade de Medicina
Instituto de Ciências Exatas e Tecnologia (ICET) Universidade Federal do Amazonas (UFAM)
Laboratório Nacional de Biociências CNPEM
Universidade Estadual Paulista (UNESP) Instituto de Biociência de Botucatu
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) UNESP
Universidade Estadual Paulista (UNESP) Faculdade de Medicina de Botucatu
Universidade Estadual Paulista (UNESP) Instituto de Biociência de Botucatu
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) UNESP
