Autor(es): Gomes-Cornélio, Ana Lívia ; Rodrigues, Elisandra Márcia ; Mestieri, Leticia Boldrin ; Falcoski, Thaís de Oliveira Rodrigues Sanzovo ; Soares, Christiane Pienna ; Guerreiro-Tanomaru, Juliane Maria ; Rossa, Carlos ; Tanomaru, Mário
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/177019
Origem: Oasisbr
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Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials.
Universidade Estadual Paulista - UNESP, Araraquara School of Dentistry, Department of Restorative Dentistry, Araraquara, SP, Brazil
Universidade Estadual Paulista - UNESP, School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, SP, Brazil
Universidade Estadual Paulista - UNESP, Araraquara School of Dentistry, Department of Diagnosis and Surgery, Araraquara, SP, Brazil
