Autor(es): Costa, Nivaldo de Azevedo ; Valeriano Simao, Luis Carlos ; dos Santos, Rogerio Adriano ; Bastos Afonso, Jose Augusto ; Fagliari, Jose Jurandir [UNESP] ; Cardoso, Elyzabeth da Cruz ; Soares, Pierre Castro ; de Mendonca, Carla Lopes
Data: 2014
Identificador Persistente: http://hdl.handle.net/11449/1772
Origem: Oasisbr
Assunto(s): Intramammary infection; Ruminants; Gram positive; Acute phase proteins; SDS-PAGE; oligoelements
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Costa N.A., Simao L.C.V., Santos R.A., Afonso J.A.B., Fagliari J.J., Cardoso E.C., Soares P.C. & Mendonca C.L. 2010. [Proteinogram and serum concentrations of copper, iron and zinc in Santa Ines ewes with Staphylococcus aureus experimentally induced mastitis.] Proteinograma e teores de cobre, ferro e zinco no soro sanguineo de ovelhas da raca Santa Ines com mastite experimental por Staphylococcus aureus. Pesquisa Veterinaria Brasileira 30(5):435-442. Clinica de Bovinos, Campus Garanhuns, Universidade Federal Rural de Pernambuco (UFRPE), Avenida Bom Pastor s/n, Boa Vista, Caixa Postal 152, Garanhuns, PE 55292-901, Brazil. E-mail: carlalopes.mendonca@gmail.comThe aim of the present study was to evaluate the effect of Staphylococcus aureus experimentally induced mastitis on proteinogram and serum concentrations of cupper, iron and zinc levels of Santa Ines primiparous ewes. The right mammary gland of ten healthy ewes was inoculated with 1,0x10(4) UFC/mL of S. aureus. Clinical examination and determination of serum concentrations of proteins by electrophoresis in polyacrylamide gel (SDS-PAGE), cupper, iron and zinc, as well plasma level of fibrinogen were measured before the inoculation (control) and 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, 120h, 132h, 168h, 180h, 288h and 336h after bacteria inoculation. All animals experimentally infected presented clinical mastitis and subsequent loss of mammary gland function. The electrophoretogram allowed the identification of 23 proteins with molecular weights (MW) ranged from 26.000 to 185.000 daltons (Da) including acute-phase proteins, IgG and IgA. A significant increase (P<0,05) in haptoglobin, ceruloplasmin, IgG and IgA concentrations was observed. Antitrypsin and acid glicoprotein concentrations did not alter. The levels of iron and zinc decreased and the cupper concentration increased. A positive correlation between plasma fibrinogen and serum ceruloplasmin (r=0.74), haptoglobin (r=0.62) and IgA (r=0.62) was also identified. Results showed the importance of ceruloplasmin and haptoglobin as acute-phase proteins in ewes with intramammary infections and confirms fibrinogen as an inflammatory marker because its high correlation with specific proteins. The alterations in the serum levels of Cu, Fe and Zn suggest the action of inflammatory mediators triggered by S. aureus.
UFRPE, Clin Bovinos, BR-55292901 Garanhuns, PE, Brazil
Universidade Federal Rural de Pernambuco (UFRPE), Programa Posgrad Ciência Vet, BR-52171030 Recife, PE, Brazil
Univ Estadual Paulista Julio de Mesquita Filho Un, Fac Ciencias Agr & Vet, Dept Clin & Cirurgia Vet, BR-14884900 Jaboticabal, SP, Brazil
Univ Fed Fluminense, Fac Med Vet, BR-24230340 Niteroi, RJ, Brazil
Universidade Federal Rural de Pernambuco (UFRPE), Dept Vet Med, BR-52171030 Recife, PE, Brazil
Univ Estadual Paulista Julio de Mesquita Filho Un, Fac Ciencias Agr & Vet, Dept Clin & Cirurgia Vet, BR-14884900 Jaboticabal, SP, Brazil
