Author(s): Ferreira, Joseane Cristina ; Penha Filho, Rafael Antonio Casarin [UNESP] ; Andrade, Leonardo Neves ; Berchieri Junior, Angelo [UNESP] ; Darini, Ana Lúcia Costa
Date: 2018
Persistent ID: http://hdl.handle.net/11449/178311
Origin: Oasisbr
Subject(s): antimicrobial resistance; E. coli; ESBL; Plasmids; pMLST
Made available in DSpace on 2018-12-11T17:29:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-08-01
The increasing presence of ESBL-producing bacteria in food-producing animals might impact on public health. In this study, ESBL-producing enterobacteria were investigated in the microbiota of chickens produced in Brazil. We detected blaCTX-M-2, blaCTX-M-8 and blaCTX-M-15 in 13 Escherichia coli isolates, within 9 different PFGE-types. Escherichia fergusonii and Klebsiella pneumoniae were found carrying blaCTX-M-2. Plasmid Inc groups found included repF, FIB, FIC, I1, Y, B/O, A/C, K and HI1. F plasmids were present in 87.5% of the isolates, however, no resistance gene was harbored in this replicon. The pMLST for IncI1 showed ST113 and the novel ST130, ST131 and ST132 harboring blaCTX-M-8. IncK plasmids carried blaCTX-M-2 in one E. coli isolate. Non-typeable plasmids carrying blaCTX-M-2 or blaCTX-M-15 had up to 260 kb. blaCTX-M-2 was also associated with class I integron and ISCR1 and blaCTX-M-8 with IS10. Overall, similar resistance elements were disseminated among a diverse population of ESBL-producing enterobacteria.
School of Pharmaceutical Sciences of Ribeirão Preto – University of São Paulo (USP)
School of Agricultural and Veterinary Sciences – São Paulo State University (UNESP)
School of Agricultural and Veterinary Sciences – São Paulo State University (UNESP)
