Author(s): de Mello Santos, Talita [UNESP] ; da Silveira, Lívia Teresa Ribeiro [UNESP] ; Rinaldi, Jaqueline Carvalho [UNESP] ; Scarano, Wellerson Rodrigo [UNESP] ; Domeniconi, Raquel Fantin [UNESP]
Date: 2018
Persistent ID: http://hdl.handle.net/11449/178747
Origin: Oasisbr
Subject(s): Androgen receptor; Antiandrogenic; Di-n-butyl-phthalate (DBP) BMP-4; Prostate development; Testosterone; α-actin
Made available in DSpace on 2018-12-11T17:31:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-04-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Prostate morphogenesis is regulated by androgens hormones and modulated by morphogenetic proteins such as Bone Morphogenetic Proteins (BMPs). This study aims to investigate the effects on prostate development in male offspring and differentiation after gestational and lactational maternal exposure to Di-n-butyl-phthalate (DBP), an important environmental contamination. Pregnant Wistar rats received 100 or 500 mg/kg of DBP (DBP100 and DBP500), by gavage, from gestation day 15 (GD15) until postnatal day 21 (PND21). The pups were euthanized on PND1 and PND21. Anogenital distance and testosterone levels decreased in animals from exposed mothers (DBP100 and 500) on PND1. A three-dimensional reconstruction model of the prostatic urethra showed reduction in the prostatic buds in the DBP500 group. AR expression and α-actin immunoreactivity decreased, and BMP-4 expression was lower on PND1 for DBP500. These results showed that DBP exposure, especially at a higher dose, delayed prostate morphogenesis by reducing the testosterone/AR axis and BMP-4 expression.
Department of Anatomy Institute of Biosciences Univi Estadual Paulista/UNESP
Department of Morphology Institute of Biosciences Univi Estadual Paulista/UNESP
Department of Anatomy Institute of Biosciences Univi Estadual Paulista/UNESP
Department of Morphology Institute of Biosciences Univi Estadual Paulista/UNESP
FAPESP: 2013/07203-4
