Descrição
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Netherlands Organisation for Scientific research (NWO)
Xanthomonas citri subsp. citri (Xcc) causes citrus canker, affecting sweet orange-producing areas around the world. The current chemical treatment available for this disease is based on cupric compounds. For this reason, the objective of this study was to design antibacterial agents. In order to do this, we analyzed the anti-Xcc activity of 36 alkyl dihydroxybenzoates and we found 14 active compounds. Among them, three esters with the lowest minimum inhibitory concentration values were selected; compounds 4 (52 M), 16 (80 M) and 28 (88 M). Our study demonstrated that alkyl dihydroxybenzoates cause a delay in the exponential phase. The permeability capacity of alkyl dihydroxybenzoates in a quarter of MIC was compared to nisin (positive control). Compound 28 was the most effective (93.8), compared to compound 16 (41.3) and compound 4 (13.9) by percentage values. Finally, all three compounds showed inhibition of FtsZ GTPase activity, and promoted changes in protofilaments, leading to depolymerization, which prevents bacterial cell division. In conclusion, heptyl dihydroxybenzoates (compounds 4, 16 and 28) are promising anti-Xcc agents which may serve as an alternative for the control of citrus canker.
Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci, Dept Chem & Environm Sci, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
Sao Paulo State Univ, Biosci Inst, Dept Biochem & Microbiol, BR-13506900 Rio Claro, SP, Brazil
Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Mol Microbiol, NL-9747 AG Groningen, Netherlands
Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci, Dept Biol, IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci, Dept Chem & Environm Sci, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
Sao Paulo State Univ, Biosci Inst, Dept Biochem & Microbiol, BR-13506900 Rio Claro, SP, Brazil
Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci, Dept Biol, IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
CNPq: 471129/2013-5
CNPq: 306251/2016-7
FAPESP: 2013/50367-8
FAPESP: 2014/18330-0
FAPESP: 2015/50162-2
FAPESP: 2017/50216-0
FAPESP: 729.004.005
Netherlands Organisation for Scientific research (NWO): 729.004.005
