Author(s): Alves, N. T. Q. ; Ximenes, R. M. ; Jorge, R. J. B. ; Silveira, J. A. M. ; Santos, J. V. A. ; Rodrigues, F. A. P. ; Costa, P. H. S. ; Xavier Jr, F. A. F. ; Evangelista, J. S. A. M. ; Hayt, A. ; Soares, V. C. G. ; Toyama, M. H. [UNESP] ; Oliveira, A. N. A. ; Araujo, R. M. ; Alves, R. S. ; Monteiro, H. S. A.
Date: 2019
Persistent ID: http://hdl.handle.net/11449/185170
Origin: Oasisbr
Subject(s): Bredemeyera floribunda; Snakeroot; Snake venom; Rutin; Antihemorrhagic; Anti-edematogenic
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Bredemeyera floribunda roots are popularly used to treat snakebites in the semiarid region of Northeast Brazil, and previous studies indicate the anti-ophidian actions of triterpenoid saponins found in its roots. To assess B. floribunda root extract (BFRE) activity against the effects of Bothrops jararacussu venom (BjuV), antiphospholipasic, antiproteolytic, antihemorrhagic, antinecrotic, and anti-edematogenic activities were investigated in mice. Phytochemical analysis revealed the presence of saponins, flavonoids, and sugars, with rutin and saccharose being the major constituents of BFRE. Acute toxicity was determined and BFRE was nontoxic to mice. Phospholipase A2 and proteolytic activities induced by BjuV were inhibited in vitro by BFRE at all concentrations tested herein. BFRE (150 mg/kg) inhibited paw edema induced by BjuV (50 mu g/animal), reducing total edema calculated by area under the curve, but carrageenan-induced paw edema was unchanged. Hemorrhagic and necrotizing actions of BjuV (50 mu g/animal) were considerably decreased by BFRE treatment. Thus, BFRE blocked the toxic actions of B. jararacussu venom despite having no anti-inflammatory activity, which points to a direct inhibition of venom's toxins, as demonstrated in the in vitro assays. The larger amounts of rutin found in BFRE may play a role in this inhibition, since 3',4'-OH flavonoids are known inhibitors of phospholipases A2.
Univ Fed Ceara, Dept Fisiol & Farmacol, Fortaleza, Ceara, Brazil
Univ Fed Pernambuco, Dept Antibiot, Recife, PE, Brazil
Univ Fed Pernambuco, Ctr Acad Vitoria, Vitoria De Santo Antao, PE, Brazil
Univ Estadual Ceara, Fac Vet, Fortaleza, Ceara, Brazil
Univ Paulista, Campus Jundiai, Jundiai, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, Sao Vicente, SP, Brazil
Univ Fed Rio Grande do Norte, Inst Quim, Natal, RN, Brazil
Univ Fed Ceara, Dept Anal Clin & Toxicol, Fortaleza, Ceara, Brazil
Univ Estadual Paulista, Inst Biociencias, Sao Vicente, SP, Brazil
