Author(s):
Lourenço, Maria Angélica Martins [UNESP] ; Braz, Mariana Gobbo [UNESP] ; Aun, Aline Garcia [UNESP] ; Pereira, Bruna Letícia Buzati [UNESP] ; Fernandes, Fábio Henrique [UNESP] ; Kazmarek, Elisa Moya [UNESP] ; Bachiega, Tatiana Fernanda [UNESP] ; Zanati, Silmeia Garcia [UNESP] ; Azevedo, Paula Schmidt [UNESP] ; Polegato, Bertha Furlan [UNESP] ; Fernandes, Ana Angélica Henrique [UNESP] ; de Paiva, Sergio Alberto Rupp [UNESP] ; Zornoff, Leonardo Antonio Mamede [UNESP] ; Minicucci, Marcos Ferreira [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/188374
Origin: Oasisbr
Subject(s): Cigarette smoke; Comet assay; DNA damage; Protein carbonyl groups
Description
Made available in DSpace on 2019-10-06T16:06:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-11-16
BACKGROUND: Oxidative stress is one potential mechanism that explain the direct effects of smoking on cardiac remodeling process. However, no study has compared different myocardial products of macromolecule oxidation after tobacco smoke exposure. Thus, the aim of this study was to investigate the lipid hydroperoxide (LH) levels, protein carbonyl concentrations and DNA damage in cardiac tissue of rats exposed to tobacco smoke. METHODS: Male Wistar rats were divided into two groups: group C (control, n = 14) composed of animals not exposed to cigarette smoke; group ETS (exposed to tobacco smoke, n = 14) composed by animals exposed to cigarette smoke. The animals were exposed to 2 month of ETS and morphological, biochemical and functional analyses were performed. RESULTS: Cardiac cotinine levels were elevated in the ETS group. In addition, the myocyte cross-sectional area was higher in the ETS group. (C = 266.6 ± 23.2 μm2 and ETS = 347.5 ± 15.1 μm2, p < 0.001). Cardiac LH was higher in the ETS group than in group C (C = 196.4 ± 51.5 nmol/g and ETS = 331.9 ± 52.9 nmol/g, p < 0.001). However, there were no between-group differences in cardiac protein carbonyl concentration or DNA damage. CONCLUSIONS: Therefore, our results suggest that, in this model, lipid damage is a good marker of oxidative damage during the cardiac remodeling process induced by 2 months of exposure to tobacco smoke.
Internal Medicine Department Botucatu Medical School UNESP - São Paulo State University
Department of Anesthesiology Botucatu Medical School UNESP - São Paulo State University
Department of Genetics Institute of Biological Sciences UNESP - São Paulo State University
Chemistry and Biochemistry Department Institute of Biological Sciences Botucatu Medical School UNESP - São Paulo State University
Departamento de Clínica Médica Faculdade de Medicina de Botucatu 18618-000, Rubião Júnior s/n
Internal Medicine Department Botucatu Medical School UNESP - São Paulo State University
Department of Anesthesiology Botucatu Medical School UNESP - São Paulo State University
Department of Genetics Institute of Biological Sciences UNESP - São Paulo State University
Chemistry and Biochemistry Department Institute of Biological Sciences Botucatu Medical School UNESP - São Paulo State University
Departamento de Clínica Médica Faculdade de Medicina de Botucatu 18618-000, Rubião Júnior s/n
