Author(s): Vasconcelos, Maria R. S. ; Vieira, Gabriela A. L. [UNESP] ; Otero, Igor V. R. [UNESP] ; Bonugli-Santos, Rafaella C. ; Rodrigues, Marili V. N. ; Rehder, Vera L. G. ; Ferro, Milene [UNESP] ; Boaventura, Sinésio ; Bacci, Maurício [UNESP] ; Sette, Lara D. [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/188816
Origin: Oasisbr
Subject(s): Artemia; Experimental design; Marine biotechnology; PAH degradation; Transcriptome
Made available in DSpace on 2019-10-06T16:20:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Marine-derived fungi are relevant genetic resources for bioremediation of saline environments/processes. Among the five fungi recovered from marine sponges able to degrade pyrene (Py) and benzo[a]pyrene (BaP), Tolypocladium sp. strain CBMAI 1346 and Xylaria sp. CBMAI 1464 presented the best removal rates of Py and BaP, respectively. Since the decrease in BaP was related to mycelial adsorption, a combined strategy was applied for the investigation of Py degradation by the fungus Tolypocladium sp. CBMAI 1346. The selected fungus was able to degrade about 95% of Py after 7 days of incubation (optimized conditions), generating metabolites different from the ones found before optimization. Metabolites and transcriptomic data revealed that the degradation occurred mainly by the cytochrome P450 pathway. Putative monooxygenases and dioxygenases found in the transcriptome may play an important role. After 21 days of degradation, no toxicity was found in the optimized culture conditions. The findings from the present study highlight the potential of marine-derived fungi to degrade environmental pollutants and convey innovative information related to the metabolism of pyrene.
Divisão de Recursos Microbianos Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas Universidade Estadual de Campinas
Departamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), 24A, 1515 - Bela Vista
Latin American Institute of Life and Nature Sciences Federal University of Latin American Integration (UNILA)
Divisão de Química Orgânica e Farmacêutica Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas Universidade Estadual de Campinas
Centro de Estudos de Insetos Sociais Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
Departamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), 24A, 1515 - Bela Vista
Centro de Estudos de Insetos Sociais Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
CNPq: 170714/2017-9
FAPESP: 2011/18769-3)
FAPESP: 2013/19486-0
FAPESP: 2016/07957-7
FAPESP: 2018/03372-0
CNPq: 303145/2016-1
