Author(s): de Siqueira-Silva, Diógenes Henrique [UNESP] ; dos Santos Silva, Amanda Pereira [UNESP] ; da Silva Costa, Raphael [UNESP] ; Senhorini, José Augusto ; Ninhaus-Silveira, Alexandre [UNESP] ; Veríssimo-Silveira, Rosicleire [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/188991
Origin: Oasisbr
Subject(s): Fish chimera; Germ cell technology; Spermatogonial proliferation; Surrogate production
Made available in DSpace on 2019-10-06T16:26:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01
We aimed to develop a simplified protocol for transplantation of Brycon orbignyanus spermatogonial stem cells (SSCs) into Astyanax altiparanae testes. Brycon orbignyanus testes were enzymatically digested and SSC purified by a discontinuous density gradient. Endogenous spermatogenesis was suppressed in A. altiparanae using busulfan or by incubation at 35 °C water, and SSCs from B. orbignyanus labeled with PKH26 were injected into their testes via the urogenital papilla. Twenty-two hours post-transplantation, labeled spermatogonia were observed in A. altiparanae tubular lumen. After 7 days, spermatogonia proliferated in the epithelium, and 21 days post-transplantation, sperm was observed in the lumen. Of surviving host fish, nearly 67% of those treated with busulfan and 85% of those held in warm water showed labeled cells in host germinal epithelium. The present study standardized, by a simple and accessible method, germ cell transplantation between sexually mature Characiformes fish species. This is the first report of xenogenic SSC transplantation in this fish order.
Campus de Ilha Solteira Departament of Biology and Animal Science L.I.NEO – Laboratório de Ictiologia Neotropical UNESP – Univ. Estadual Paulista, Avenida Brasil Centro, 56
Campus de São José do Rio Preto Post-Graduation Program in Animal Biology UNESP – Univ. Estadual Paulista, Rua Cristovão Colombo, Jardim Nazareth, 2265
Campus de Ilha Solteira Departamento de Biologia e Zootecnia L.I.NEO - Laboratório de Ictiologia Neotropical UNESP –Univ. Estadual Paulista, Rua Monção, 226, Zona Norte
CEPTA-ICMBIO - National Center for Research and Conservation of Continental Fish Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemesio Pereira de Godoy
Campus de Ilha Solteira Departament of Biology and Animal Science L.I.NEO – Laboratório de Ictiologia Neotropical UNESP – Univ. Estadual Paulista, Avenida Brasil Centro, 56
Campus de São José do Rio Preto Post-Graduation Program in Animal Biology UNESP – Univ. Estadual Paulista, Rua Cristovão Colombo, Jardim Nazareth, 2265
Campus de Ilha Solteira Departamento de Biologia e Zootecnia L.I.NEO - Laboratório de Ictiologia Neotropical UNESP –Univ. Estadual Paulista, Rua Monção, 226, Zona Norte
