Author(s): De Paula, Carolina Lechinski [UNESP] ; Silva, Rodrigo Otávio Silveira ; Hernandes, Rodrigo Tavanelli [UNESP] ; Júnior, Geraldo De Nardi ; Babboni, Selene Daniela ; Guerra, Simony Trevizan [UNESP] ; Listoni, Fernando José Paganini [UNESP] ; Giuffrida, Rogério ; Takai, Shinji ; Sasaki, Yukako ; Ribeiro, Márcio Garcia [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/189454
Origin: Oasisbr
Made available in DSpace on 2019-10-06T16:41:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01
Rhodococcus equi is responsible for infections in multiple-host animals. In humans, the prevalence of rhodococcus has increased worldwide and represents an emergent risk. R. equi is a soil-borne opportunistic bacterium isolated from feces of a wide variety of domestic species, except cats; thus, there is no known potential risk of its transmission from humans. Here, the mono- and cooccurrence of Rhodococcus equi and other bacteria and selected virulence markers were investigated in feces of nondiarrheic cats from urban (n=100) and rural (n=100) areas. Seven (7/200=3.5%) R. equi isolates were recovered in ceftazidime, novobiocin, and cycloheximide (CAZ-NB) selective media, exclusively of cats from three distinct farms (p=0.01), and these cats had a history of contact with horses and their environment (p=0.0002). None of the R. equi isolates harbored hosted-adapted plasmid types associated with virulence (pVAPA, pVAPB, and pVAPN). One hundred seventy-five E. coli isolates were identified, and 23 atypical enteropathogenic E. coli (aEPEC), 1 STEC (Shiga-toxin producing E. coli), and 1 EAEC (enteroaggregative E. coli) were detected. Eighty-six C. perfringens type A isolates were identified, and beta-2 and enterotoxin were detected in 21 and 1 isolates, respectively. Five C. difficile isolates were identified, one of which was toxigenic and ribotype 106. The main cooccurring isolates in cats from urban areas were E. coli and C. perfringens A (26/100=26%), E. coli and C. perfringens type A cpb2+ (8/100=8%), and aEPEC (eae+/escN+) and C. perfringens type A (5/100=5%). In cats from farms, the main cooccurring isolates were E. coli and C. perfringens type A (21/100=21%), E. coli and C. perfringens type A cpb2+ 8/100=8%), and E. coli and R. equi (4/100=4%). We identified, for the first time, R. equi in nondiarrheic cats, a finding that represents a public health issue because rhodococcus has been reported in both immunosuppressed and immunocompetent humans, particularly people living with HIV/AIDS.
Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-São Paulo State University
Department of Preventive Veterinary Medicine Federal University of Minas Gerais UFMG
Department of Microbiology and Immunology Biosciences Institute UNESP
Technology Faculty FATEC
Paulista University UNIP Sao Jose Dos Campos
School of Veterinary Medicine University of Oeste Paulista UNOESTE
School of Veterinary Medicine Kitasato University
Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-São Paulo State University
Department of Microbiology and Immunology Biosciences Institute UNESP
