Author(s): dos Anjos, Carolina ; Sellera, Fábio Parra ; Gargano, Ronaldo Gomes ; Lincopan, Nilton ; Pogliani, Fabio Celidonio ; Ribeiro, Marcio Garcia [UNESP] ; Jagielski, Tomasz ; Sabino, Caetano Padial
Date: 2019
Persistent ID: http://hdl.handle.net/11449/189702
Origin: Oasisbr
Subject(s): Algae decontamination; Antimicrobial Photodynamic Therapy; Bovine protothecosis; Photoinactivation; Prototheca blaschkeae; Prototheca zopfii; Veterinary medicine
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Prototheca spp. are pathogenic algae with important zoonotic potential. Most importantly, these algae often infect dairy cattle. Since there is no effective therapy against the algae, the standard recommendation is the disposal or culling of infected cows to avoid outbreaks. This study investigated the ability of blue light to inactivate pathogenic Prototheca species. Blue LED light (λ = 410 nm) was used to inactivate in vitro suspensions of P. zopfii genotypes 1 and 2, and P. blaschkeae. Our results showed that blue light irradiation induced a strain-specific dose-dependent algicidal effect against all tested strains. P. zopfii genotype 1, was more sensitive than genotype 2 and P. blaschkeae was the most tolerant. Even though we observed different inactivation kinetics, all strains presented significant photoinactivation levels within feasible procedure periods. Therefore, we conclude that blue light irradiation offers promising potential for the development of novel technologies that control contaminations and infections caused by Prototheca spp.
Department of Internal Medicine School of Veterinary Medicine and Animal Science University of Sao Paulo
Department of Microbiology Institute for Biomedical Sciences University of Sao Paulo
Department of Clinical and Toxicological Analysis School of Pharmaceutical Sciences University of Sao Paulo
Department of Veterinary Hygiene and Public Health Sao Paulo State University (UNESP)
Department of Applied Microbiology Institute of Microbiology Faculty of Biology University of Warsaw
BioLambda Scientific and Commercial LTD
Department of Veterinary Hygiene and Public Health Sao Paulo State University (UNESP)
FAPESP: 2016/25095-2
