Autor(es): Souza, Luanda B. F. C. ; Silva-Rocha, Walicyranison P. ; Ferreira, Magda R. A. ; Soares, Luiz Alberto L. ; Svidzinski, Terezinha I. E. ; Milan, Eveline P. ; Pires, Regina H. [UNESP] ; Almeida, Ana Marisa Fusco [UNESP] ; Mendes-Giannini, Maria Jos S. [UNESP] ; Chaves, Guilherme Maranhão
Data: 2019
Identificador Persistente: http://hdl.handle.net/11449/189757
Origem: Oasisbr
Assunto(s): Candida spp.; Eugenia uniflora; Kidney transplant recipients; Oral candidiasis; Virulence factors
Made available in DSpace on 2019-10-06T16:51:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-09-20
Made available in DSpace on 2019-10-06T16:51:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-09-20
This study evaluated the influence of the extract of Eugenia uniflora in adhesion to human buccal epithelial cells (HBEC) biofilm formation and cell surface hydrophobicity (CSH) of Candida spp. isolated from the oral cavity of kidney transplant patients. To evaluate virulence attributes in vitro, nine yeasts were grown in the presence and absence of 1000 g/mL of the extract. Adhesion was quantified using the number of Candida cells adhered to 150 HBEC determined by optical microscope. Biofilm formation was evaluated using two methodologies: XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) and crystal violet assay, and further analyzed by electronic scan microscopy. CSH was quantified with the microbial adhesion to hydrocarbons test. We could detect that the extract of E. uniflora was able to reduce adhesion to HBEC and CSH for both Candida albicans and non-Candida albicans Candida species. We also observed a statistically significant reduced ability to form biofilms in biofilm-producing strains using both methods of quantification. However, two highly biofilm-producing strains of Candida tropicalis had a very large reduction in biofilm formation. This study reinforces the idea that besides growth inhibition, E. uniflora may interfere with the expression of some virulence factors of Candida spp. and may be possibly applied in the future as a novel antifungal agent.
Departamento de Análises Clínicas e Toxicológicas Universidade Federal Do Rio Grande Do Norte-UFRN, Rua Gal, Gustavo Cordeiro de Faria, S/N
Departamento de Ciências Farmacêuticas Universidade Federal de Pernambuco-UFPE
Departamento de Análises Clínicas Universidade Estadual de Maringá-UEM, Avenida Colombo, 5790
Departamento de Infectologia Universidade Federal Do Rio Grande Do Norte-UFRN, Rua Conego Monte, 110
Laboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista-UNESP
Laboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista-UNESP
