Autor(es): Oliveira, Lucas Pires Garcia [UNESP] ; Conte, Fernanda Lopes [UNESP] ; de Oliveira Cardoso, Eliza [UNESP] ; Conti, Bruno José [UNESP] ; Santiago, Karina Basso [UNESP] ; de Assis Golim, Marjorie [UNESP] ; da Silva Feltran, Geórgia [UNESP] ; Zambuzzi, Willian Fernando [UNESP] ; Sforcin, José Maurício [UNESP]
Data: 2019
Identificador Persistente: http://hdl.handle.net/11449/189946
Origem: Oasisbr
Assunto(s): Chemotherapy; Geopropolis; Immunomodulation; Monocytes
Made available in DSpace on 2019-10-06T16:57:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-15
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Aims: Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes. Main methods: Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α IL-1β IL-6 and IL-10), intracellular pathways (NF-κB and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H 2 O 2 ) production were analyzed. Key findings: Data showed that doxorubicin + geopropolis diminished IL-6 secretion, stimulated TNF-α and IL-10 production, TLR-4 and CD80 expression, NF-κB and autophagy pathway, as well as the bactericidal activity. Significance: Our findings revealed a new chemotherapeutic approach using doxorubicin simultaneously with geopropolis without affecting human monocytes viability and exerting immunomodulatory effects, favoring cell functions. While doxorubicin altered some immunological parameters, the addition of geopropolis compensated some changes.
São Paulo State University (UNESP) Institute of Biosciences
Botucatu Blood Center School of Medicine UNESP
São Paulo State University (UNESP) Institute of Biosciences
Botucatu Blood Center School of Medicine UNESP
FAPESP: 2016/02249-4
