Author(s): Artal, Mariana Coletty ; Pereira, Karina Danielle [UNESP] ; Luchessi, Augusto Ducati [UNESP] ; Okura, Vagner Katsumi ; Henry, Theodore Burdick ; Marques-Souza, Henrique ; Umbuzeiro, Gisela de Aragao
Date: 2020
Persistent ID: http://hdl.handle.net/11449/195337
Origin: Oasisbr
Subject(s): Amphipod; Gene expression; Glutathione-S-Transferase; RNA-Seq; RT-qPCR
Made available in DSpace on 2020-12-10T17:31:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Analysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology. (C) 2020 Elsevier Ltd. All rights reserved.
Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo, Brazil
Univ Estadual Campinas, Sch Technol, BR-13484332 Limeira, SP, Brazil
Univ Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, SP, Brazil
Sao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil
Univ Estadual Campinas, Life Sci Core Facil LaCTAD, BR-13083886 Campinas, SP, Brazil
Heriot Watt Univ, Sch Energy Geosci Infrastruct & Soc, Inst Life & Earth Sci, Edinburgh EH14 4AS, Midlothian, Scotland
Univ Tennessee, Ctr Environm Biotechnol, 676 Dabney Hall,1416 Circle Dr, Knoxville, TN 37996 USA
Univ Estadual Campinas, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP, Brazil
Sao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil
FAPESP: 2014/08829-7
CNPq: 400362/2014-7
CAPES: 001
