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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Brazilian Agricultural Research Corporation
PROPE / Unesp
To evaluate follicular dynamics, there was assessment of superovulatory response and in vivo embryo production in ewes treated with relatively smaller doses of exogenous pFSH than typically used in combination with a dose of eCG at the beginning of the gonadotropin treatment period. Santa Ines ewes (n = 24) were randomly divided into three groups, based on mg dose of pFSH administered: G200 (n = 8), G133 (n = 8) and G100 (n = 8) in eight decreasing doses at 12-h intervals. All ewes were treated with 300 IU of eCG concomitantly starting with first pFSH administration. Ovulatory follicular dynamics and follicular wall vascularization (FWV) were evaluated using a B-mode and color Doppler ultrasonic machine, respectively. Superovulatory response and embryo production were evaluated 6 days after estrous detection. In the G200 group, the preovulatory follicle size (PFS) were less (P < 0.05), ovulation time later (P < 0.05), and PFS rate greater (P < 0.05); while in the G100 group ovulation rate, and number and percentage of unfertilized eggs were greater (P < 0.05) than in the G133 group (P < 0.05). Number and percentage of viable embryos were greater in the G200 and G100 compared to G133 group (P < 0.05). The dose of 100 mg of FSH was as efficacious as the traditional dose of 200 mg, in combination with a dose of eCG, for superovulatory response and viable embryo production but there was a greater percentage of unfertilized eggs with this treatment.
Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
Fed Univ Jequitinhonha & Mucuri Valleys, Inst Agr Sci, Av Joao Narciso 1380, BR-38610000 Unai, MG, Brazil
Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
Brazilian Agr Res Corp Embrapa Dairy Cattle, BR-27640000 Valenca, RJ, Brazil
Univ Fed Santa Maria, Cidade Univ, Dept Clin Large Anim, Av Roraima 1000,Cidade Univ, BR-97105900 Santa Maria, RS, Brazil
Brazilian Agr Res Corp Embrapa Goats & Sheep, BR-36155000 Coronel Pacheco, MG, Brazil
Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Preventat Vet Med & Anim Reprod, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
FAPESP: 2014/04614-6
Brazilian Agricultural Research Corporation: 02.13.06.026.00.03
PROPE / Unesp: TC1288 / 2015
