Author(s): Sant' Anna, Debora ; Sampaio, Jorge Luiz Mello ; Sommaggio, Lais Roberta Deroldo [UNESP] ; Mazzeo, Dania Elisa Christofoletti [UNESP] ; Marin-Morales, Maria Aparecida [UNESP] ; Marson, Fernando Augusto Lima ; Levy, Carlos Emilio
Date: 2020
Persistent ID: http://hdl.handle.net/11449/196561
Origin: Oasisbr
Subject(s): 16S rRNA; Advenella; Castellaniella; Kaistia; Pusillimonas; Sphingobacterium
Made available in DSpace on 2020-12-10T19:48:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01
Our aim was to identify less common non-fermenting gram-negative rods during the bioremediation process. Five genera were found: Advenella, Castellaniella, Kaistia, Pusillimonas and Sphingobacterium, for a total of 15 isolates. Therefore, we evaluated the applicability of four methods currently available for bacteria identification: (1) conventional biochemical methods, (2) the VITEK (R)-2 system, (3) MALDI-TOF mass spectrometry and (4) 16S rRNA gene sequencing. The biochemical methods and the VITEK (R)-2 system were reliable only for the Sphingobacterium isolate and solely at the genus level. Both MALDI-TOF mass spectrometry platforms (Bruker and VITEK (R) MS) did not achieve reliable identification results for any of these genera. 16S rRNA gene sequencing identified eight isolates to the species level but not to the subspecies level, when applicable. The remaining seven isolates were reliably identified through 16S rRNA gene sequencing to the genus level only. Our findings suggest that the detection and identification of less common genera (and species) that appeared at certain moments during the bioremediation process can be a challenge to microbiologists considering the most used techniques. In addition, more studies are required to confirm our results.
Univ Estadual Campinas, Fac Med Sciences, Dept Clin Pathol, MicroBiol Lab, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil
Barretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Villela, 1331, Barretos, SP, Brazil
MicroBiol Sect, Centers Diagnost Med, . Gen Waldomiro Lima 508, BR-04344-07 Sao Paulo, Brazil
Univ Sao Paulo, Sch Pharm, Toxicol Dept, Clin Anal, . Prof Lineu Prestes, 580, BR-05508-00 Butanta, SP, Brazil
Sao Paulo State Univ, Inst Biosciences, Dept Biol, . 24, 1515, Bela Vista, BR-13506-90 Rio Claro, SP, Brazil
Sao Paulo State Univ, Inst Chem, Dept Analyt Chem, Rua Prof Francisco Degni, 55, BR-14800-06 Araraquara, SP, Brazil
Univ Estadual Campinas, Fac Med Sciences, Dept Pediat, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil
Univ Estadual Campinas, Fac Med Sciences, Ctr Pediat Invest, Lab Pulm Physiol, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil
Univ Estadual Campinas, Fac Med Sciences, Dept Med Genet, Gen Med, Rua Tessalia Vieira Camargo, 126, BR-13083-88 Campinas, SP, Brazil
Sao Francisco Univ, PostGrad Program Hlth Sci, Ave Sao Francisco Assis, 218, BR-12916-40 Braganca Paulista, SP, Brazil
Sao Paulo State Univ, Inst Biosciences, Dept Biol, . 24, 1515, Bela Vista, BR-13506-90 Rio Claro, SP, Brazil
Sao Paulo State Univ, Inst Chem, Dept Analyt Chem, Rua Prof Francisco Degni, 55, BR-14800-06 Araraquara, SP, Brazil
