Autor(es): Engel, Kathrin M. ; Dzyuba, Viktoriya ; Ninhaus-Silveira, Alexandre [UNESP] ; Verissimo-Silveira, Rosicleire [UNESP] ; Dannenberger, Dirk ; Schiller, Juergen ; Steinbach, Christoph ; Dzyuba, Borys
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/196727
Origem: Oasisbr
Assunto(s): lipidomics; sperm; freshwater fish; mass spectrometry; thin-layer chromatography; fertilization mode
Made available in DSpace on 2020-12-10T19:54:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01
Czech Science Foundation
Ministry of Education, Youth and Sports of the Czech Republic-project CENAKVA
project Biodiversity
Grant Agency of the University of South Bohemia in Ceske Budejovice
German Research Foundation
The lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization.
Univ Leipzig, Med Fac, Inst Med Phys & Biophys, Hartelstr 16-18, D-04107 Leipzig, Germany
Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Zatisi 728-2, Vodnany 38925, Czech Republic
Sao Paulo State Univ, Fac Engn, Dept Biol & Zootechny, Neotrop Ichthyol Lab LINEO, Moncao St 226, BR-15385000 Ilha Solteira, SP, Brazil
Leibniz Inst Farm Anim Biol, Inst Muscle Biol & Growth, Lipid Metab & Muscular Adaptat Workgrp, Wilhelm Stahl Allee 2, D-18196 Dummerstorf, Germany
Sao Paulo State Univ, Fac Engn, Dept Biol & Zootechny, Neotrop Ichthyol Lab LINEO, Moncao St 226, BR-15385000 Ilha Solteira, SP, Brazil
Czech Science Foundation: 16-03754S
Ministry of Education, Youth and Sports of the Czech Republic-project CENAKVA: LM2018099
project Biodiversity: CZ.02.1.01./0.0/0.0/16_025/0007370
Grant Agency of the University of South Bohemia in Ceske Budejovice: 125/2016/Z
German Research Foundation: DFG SCHIJ 476/12-2
