Author(s): Branco-de-Almeida, Luciana S. ; Franco, Gilson C. N. ; Castro, Myrella L. ; Vieira, Mayana S. ; Galvao-Moreira, Leonardo ; Cortelli, Sheila C. ; Anbinder, Ana L. [UNESP] ; Kawai, Toshihisa ; Rosalen, Pedro L.
Date: 2020
Persistent ID: http://hdl.handle.net/11449/196943
Origin: Oasisbr
Subject(s): bone resorption; collagen; desipramine; inflammation; periodontal diseases
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR, USA)
Maranhao State Research Foundation (FAPEMA, Brazil)
Background Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. Methods A total of 60 male Wistar rats were randomly assigned into three groups: 1) control: rats without ligature treated with vehicle (saline); 2) ligature: rats with ligature-induced periodontitis treated with vehicle; 3) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/d in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1 beta, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Results Alveolar bone loss was significantly reduced in the ligature + desipramine group (P < 0.05), whereas gingival collagen degradation was like the ligature group (P > 0.05). Desipramine administration downregulated mRNA expressions of IL-1 beta, iNOS, COX-2, and TIMP-1 when compared to vehicle alone in the ligature group (P < 0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P > 0.05); however, MMP-9 activity was lower in the group treated with desipramine (P < 0.05). Conclusion Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis.
Univ Fed Maranhao, Post Grad Program Dent, Sao Luis, Maranhao, Brazil
Univ Estadual Ponta Grossa, Dept Gen Biol, Ponta Grossa, Parana, Brazil
Fac Sci Tocantins, Araguaina, Tocantins, Brazil
Univ Ceuma, Post Grad Program Dent, Sao Luis, Maranhao, Brazil
Univ Fed Maranhao, Sch Med, Sao Luis, Maranhao, Brazil
Univ Taubate, Nucleus Periodontal Res, Taubate, SP, Brazil
Sao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil
Nova Southeastern Univ, Coll Dent Med, Ft Lauderdale, FL 33314 USA
Univ Fed Alfenas, Biol Sci Grad Program, Rua Gabriel Monteiroda Silva 700,Predio E Sala, BR-37130001 Alfenas, MG, Brazil
Sao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil
FAPESP: 2008/00566-6
CAPES: 4073/08-8
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR, USA): R15 DE027851
Maranhao State Research Foundation (FAPEMA, Brazil): 1818/2012
