Author(s): Franco Oliveira, Maria Emilia [UNESP] ; Fonseca, Jeferson Ferreira ; Russiano Vicente, Wilter Ricardo [UNESP] ; Rodrigues, Naiara Nantes [UNESP] ; Vergani, Gabriel Brun [UNESP] ; Goncalves Souza-Fabjan, Joanna Maria ; Jamieson, Mark ; Cristescu, Ada ; Murawski, Maciej ; Bartlewski, Pawel Mieczyslaw
Date: 2020
Persistent ID: http://hdl.handle.net/11449/197126
Origin: Oasisbr
Subject(s): Sheep; Superovulation; Estradiol benzoate; Doppler ultrasonography; Ovarian artery
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Department of Biomedical Sciences, U of G
Embrapa Goats and Sheep
Nineteen ewes received 200 mg of pFSH administered in eight decreasing doses from Days 1 to 4, starting three days before CIDR (R) device removal. Ten ewes received an injection of 350 mu g of estradiol benzoate at CIDR (R) device insertion (Group E) and nine animals served as controls (Group C). B-mode and spectral Doppler ultra-sonographic examinations were performed daily throughout superovulatory treatment to enumerate ovarian antral follicles and to determine ovarian blood flow indices, respectively. There were no differences (P > 0.05) in superovulatory responses between left and right ovaries/uterine horns or the two groups of animals. End-diastolic velocity (EDV) and mean velocity (Vm) values were greater (P < 0.05) on Days 1 and 2, and peak systolic velocity (SVp) was greater (P < 0.05) on Day 3 in Group C than in Group E. In Group E 15 correlations was recorded among indices (SVp, Vm, EDV, flow velocity integral-FVI, and pulsatility index-PI) and follicles numbers in different size classes on Days 1, 2 and 4, and seven correlations among indices (SVp, EDV, Vm, and vascular resistance index-RI) and superovulatory/embryo results (numbers of regressing corpora lutea, numbers/percentages of degenerated embryos and viability rates) on Days 1, 2 and 3. In Group C, there were three correlations among EDV and RI and medium-sized/large follicle numbers on Days 1 and 3, and five correlations among indices (EDV, RI and PI) and superovulatory/embryo results (numbers of luteinized unovulated follicles, degenerated embryos and unfertilized eggs) on Days 2 or 4. There was a lack of consistency in the velocimetric correlates of antral follicle numbers and superovulatory responses between the left and right side. Therefore, the usefulness of ovarian arterial indices to predict ovine superovulatory outcomes remains equivocal and requires further confirmatory studies.
Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, BR-14884900 Jaboticabal, SP, Brazil
Brazilian Agr Res Corp, Embrapa Goats & Sheep, BR-36155000 Coronel Pacheco, MG, Brazil
Fluminense Fed Univ, Fac Vet Med, BR-24230340 Niteroi, RJ, Brazil
Univ Guelph, Dept Biomed Sci, Ontario Vet Coll, Guelph, ON N1G 2W1, Canada
Agr Univ Krakow, Dept Anim Biotechnol, Krakow, Poland
Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, BR-14884900 Jaboticabal, SP, Brazil
CNPq: 150896/2012-3
Embrapa Goats and Sheep: Superovi - 22.13.06.026.00.03
