Autor(es): Muñoz, Vitor Rosetto ; Gaspar, Rafael Calais ; Minuzzi, Luciele Guerra ; dos Santos Canciglieri, Raphael ; da Silva, Adelino Sanchez Ramos ; de Moura, Leandro Pereira [UNESP] ; Cintra, Dennys Esper ; Ropelle, Eduardo Rochete ; Pauli, José Rodrigo
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/198042
Origem: Oasisbr
Assunto(s): Aging; Insulin signaling; Physical exercise; Rho-kinase; Skeletal muscle
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
There is a gap in the knowledge regarding the regulation of glucose uptake in skeletal muscle during the development of insulin resistance in the elderly. Rho-Kinase (Rock) signaling has been demonstrated as a crucial mechanism related to glucose metabolism and insulin sensitivity in skeletal muscle. This kinase is involved in the insulin receptor substrate 1 (IRS1) phosphorylation, leading to glucose uptake stimulation in the skeletal muscle; however, the mechanisms elucidating the role of Rock regulation in the context of advanced ages are still limited. In this study, we submitted old Fischer 344 rats to short-term treadmill physical exercise protocol (5 days) and evaluated the glucose tolerance and proteins involved with Rock/insulin signaling in the skeletal muscle. Compared to young rats, the old rats showed glucose intolerance, hyperinsulinemia, and decreased phosphorylation in the proteins related to the insulin signaling pathway in the skeletal muscle, without changes in body mass and adiposity. Otherwise, when these rats were submitted to physical exercise, it was found decreased fasting glucose, higher glucose tolerance, decreased insulinemia, and upregulation of Rock2/pIRS1/pAkt/pGSK3β/GLUT4 pathway in the skeletal muscle. In summary, the aging process did not change Rock signaling, but the physical exercise was able to increase Rock2 content and insulin signaling pathway in the skeletal muscle. This finding suggests the benefic role of physical exercise to advanced ages, promoting insulin-sensitive effects with Rho-kinase contribution.
Laboratory of Molecular Biology of Exercise University of Campinas (UNICAMP)
Center for Neuroscience and Cell Biology (CNC.IBILI) University of Coimbra
Postgraduate Program in Rehabilitation and Functional Performance Ribeirão Preto Medical School USP
School of Physical Education and Sport of Ribeirão Preto University of São Paulo (USP)
OCRC - Obesity and Comorbidities Research Center University of Campinas (UNICAMP)
Postgraduate Program in Motor Science - São Paulo State University (UNESP)
CEPECE - Center of Research in Sport Sciences School of Applied Sciences University of Campinas (UNICAMP)
Exercise Cell Biology Lab University of Campinas (UNICAMP)
Laboratory of Nutritional Genomics University of Campinas (UNICAMP)
Postgraduate Program in Motor Science - São Paulo State University (UNESP)
CAPES: 001
FAPESP: 2015/26000-2
FAPESP: 2016/18488-8
FAPESP: 2018/20872-6
CNPq: 306535/2017-3
CNPq: 442542/2014-3
