Author(s): Gardizani, Taiane Priscila [UNESP] ; Della Coletta, Amanda Manoel [UNESP] ; Romagnoli, Graziela Gorete [UNESP] ; Puccia, Rosana ; Serezani, Ana Paula Moreira ; De Campos Soares, Ângela Maria Victoriano [UNESP] ; Dias-Melicio, Luciane Alarcão [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/199812
Origin: Oasisbr
Made available in DSpace on 2020-12-12T01:49:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01
The glycoprotein gp43 is the major antigenic/diagnostic component of Paracoccidioides brasiliensis, one of the etiologic agents of paracoccidioidomycosis (PCM). Gp43 has protective roles in mice, but due to adhesive properties, this glycoprotein has also been associated with immune evasion mechanisms. The present study evaluated gp43 interaction in vitro with Toll-like receptors 2 and 4 (TLR2 and TLR4) present in polymorphonuclear neutrophils (PMNs) from healthy human individuals and the consequent modulation of the immune response through the expression and release of cytokines and eicosanoids. PMNs were incubated in the absence or presence of monoclonal antibodies anti-TLR2 and anti-TLR4 (individually or in combination) before gp43 stimulation. Then, PMNs were analyzed for the expression of both surface receptors and the detection of intracytoplasmic IL-17A and IL-4 using flow cytometry, while the production of PGE2, LTB4, IL-6, IL-10, IL-12, IFN-γ, and TNF-α was evaluated in the supernatants by enzyme-linked immunosorbent assay (ELISA). Our results showed that gp43 increased TLR2 and TLR4 expression by PMNs and induced PGE2 and IL-17A via TLR4 and TLR2, respectively. Thus, our data suggest that gp43 from P. brasiliensis might modulate host susceptibility to the fungal infection by affecting PGE2 and IL-17A production.
Laboratory of Immunopathology and Infectious Agents (LIAI) Experimental Research Unit (UNIPEX) Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior
Department of Medicine University Center of Adamantina, Av. Francisco Bellusci, 1000, Distrito Industrial Otavio Gacazzi
Department of Microbiology and Immunology Institute of Biosciences (IB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior
Departamento de Microbiologia Imunologia e Parasitologia Escola Paulista de Medicina-Universidade Federal de São Paulo, Rua Botucatu, 862
Division of Allergy Pulmonary and Critical Care Medicine Department of Medicine Vanderbilt University Medical Center
Department of Pathology Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior
Laboratory of Immunopathology and Infectious Agents (LIAI) Experimental Research Unit (UNIPEX) Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior
Department of Microbiology and Immunology Institute of Biosciences (IB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior
Department of Pathology Medical School of Botucatu (FMB) São Paulo State University (UNESP), Av. Professor Montenegro, s/n, Distrito de Rubião Júnior
