Autor(es): Chaves, Cláudia Elizabeth Volpe ; de Oliveira, Sandra Maria do Valle Leone ; Venturini, James ; Grande, Antonio Jose ; Sylvestre, Tatiane Fernanda [UNESP] ; Mendes, Rinaldo Poncio [UNESP] ; Paniago, Anamaria Mello Miranda
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/200170
Origem: Oasisbr
Made available in DSpace on 2020-12-12T01:59:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Chronic pulmonary aspergillosis (CPA) is a slow and progressive disease that develops in preexisting lung cavities of patients with tuberculosis sequelae, and it is associated with a high mortality rate. Serological tests such as double agar gel immunodiffusion test (DID) or counterimmunoelectrophoresis (CIE) test have been routinely used for CPA diagnosis in the absence of positive cultures. However, these tests have been replaced with enzyme-linked immunoassay (ELISA) and, a variety of methods. This systematic review compares ELISA accuracy to reference test (DID and/or CIE) accuracy in CPA diagnosis. It was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The study was registered in PROSPERO under the registration number CRD42016046057. We searched the electronic databases MEDLINE (PubMed), EMBASE (Elsevier), LILACS (VHL), Cochrane library, and ISI Web of Science. Gray literature was researched using Google Scholar and conference abstracts. We included articles with patients or serum samples from patients with CPA who underwent two serological tests: ELISA (index test) and IDD and/or CIE (reference test). We used the test accuracy as a result. Original articles were considered without a restriction of date or language. The pooled sensitivity, specificity, and summary receiver operating characteristic curves were estimated. We included 14 studies in the review, but only four were included in the meta-analysis. The pooled sensitivities and specificities were 0.93 and 0.97 for the ELISA test. These values were 0.64 and 0.99 for the reference test (DID and/or CIE). Analyses of summary receiver operating characteristic curves yielded 0.99 for ELISA and 0.99 for the reference test (DID and/or CIE). Our meta-analysis suggests that the diagnostic accuracy of ELISA is greater than the reference tests (DID and/or CIE) for early CPA detection.
Graduate Program in Infectious and Parasitic Diseases Federal University of Mato Grosso do Sul
Regional Hospital of Mato Grosso do Sul Campo Grande
State University of Mato Grosso do Sul Campo Grande
Tropical Diseases Department Faculdade de Medicina de Botucatu Universidade Estadual Paulista (UNESP)
Tropical Diseases Department Faculdade de Medicina de Botucatu Universidade Estadual Paulista (UNESP)
