Author(s): Dos Santos, Catarina [UNESP] ; Mizobucchi, Andressa Lie [UNESP] ; Escaramboni, Bruna [UNESP] ; Lopes, Bruno Pereira [UNESP] ; Angolini, Celio Fernando Figueiredo ; Eberlin, Marcos Nogueira ; De Toledo, Karina Alves [UNESP] ; Núñez, Eutimio Gustavo Fernández
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200584
Origin: Oasisbr
Subject(s): Antioxidant; Antiproliferative activities; Eugenia punicifolia; Extraction; Mixture design
Made available in DSpace on 2020-12-12T02:10:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-27
Eugenia punicifolia (Kunth) D. C. (Myrtaceae) has been showing interesting biological activities in the literature which was correlated to its phenolic compounds. In the sense of a better recovering of phenolics with the best antioxidant and antiproliferative activities, an extraction, based on multivariate analytical approach, was developed from E. punicifolia leaves. The different extractor solvents (ethanol, methanol and water) and their binary and ternary combinations were evaluated using a simplex-centroid mixture design and surface response methodology. The optimized crude extracts were investigated for phenol and flavonoid content and compared to their antioxidant (EC50) and antiproliferative properties against HEp-2 (cell line derived from the oropharyngeal carcinoma) and mononuclear viability cells. Ethanolic extracts showed the best phenolic content with the highest antioxidant activity and moderated activity antiproliferative to HEp-2. ESI-QTOF-MS revealed the presence of quercetin and myricetin derivatives, which was correlated to activities tested. Then, simplex-centroid design allowed us to correlate the Eugenia punicifolia biological activities with the extracts obtained from solvent different polarity mixtures.[Figure not available: see fulltext.]
Laboratory of Chemistry of UNESP-Assis (LAQUA) Department of Biological Sciences Faculty of Sciences and Letters University of São Paulo State (UNESP), Sto Antonio Ave
Department of Biotechnology Faculty of Sciences and Letters University of São Paulo State (UNESP)
Laboratory of Physiopathology of Innate Immunity Department of Biological Sciences Faculty of Sciences and Letters University of São Paulo State (UNESP)
Mass Spectrometry and Chemical Ecology Laboratory (MS-Cell) Center for Natural and Human Sciences Federal University of ABC (UFABC)
Thomson Mass Spectrometry Laboratory Institute of Chemistry University of Campinas (UNICAMP)
Mackenzie Presbyterian University Mackenzie Research Nucleus in Science Faith and Society, Maria Antônia Street, 163 Room 44, Vila Buarque
School of Arts Sciences and Humanities University of São Paulo (EACH-USP), Arlindo Béttio Street, 1000 - Vila Guaraciaba
Laboratory of Chemistry of UNESP-Assis (LAQUA) Department of Biological Sciences Faculty of Sciences and Letters University of São Paulo State (UNESP), Sto Antonio Ave
Department of Biotechnology Faculty of Sciences and Letters University of São Paulo State (UNESP)
Laboratory of Physiopathology of Innate Immunity Department of Biological Sciences Faculty of Sciences and Letters University of São Paulo State (UNESP)
