Author(s): Silva, Larissa Pinheiro ; Paciello, Mauricio Oviedo ; Aviz Teixeira, Wéllida Patricia ; Rivas, Açucena Veleh ; Agular, Raimundo Wagner Souza ; Cangussu, Alex Sander Rodrigues ; Barbosa, Luiz Carlos Bertucci ; Marchetto, Reinaldo [UNESP] ; Giunchetti, Rodolfo Cordeiro ; Viana, Kelvinson Fernandes
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200862
Origin: Oasisbr
Subject(s): adjuvant; bioinformatics; Gp63; leishmaniasis; peptide; vaccine
Made available in DSpace on 2020-12-12T02:18:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Aims: This study aimed to evaluate the toxicity and humoral and cellular immune response of three heterologous vaccines against Leishmania infantum, yet containing synthetic peptides from Leishmania major in the experimental model in hamsters. Methods and Results: Through bioinformatics analyses, two Leishmania major Gp63 peptides were predicted and selected for vaccine formulations. Hamsters were divided into four groups, with each group receiving doses of three vaccine formulations containing HLA-DR1 or HLA-A2 peptides plus MontanideTM or both associated with the adjuvant. The animals received three vaccine doses and were evaluated for toxicity after each dose, in addition to being analysed for the production of antibodies and lymphoproliferation on day 211 after the last vaccine dose. Peptides predicted in association with oily adjuvant induced a humoral response and strong lymphoproliferation to Leishmania infantum antigen-specific stimulation.
Laboratory of Biomolecules and Vaccines Postgraduate Program in Biotechnology Agrarian Sciences and Technologic Department Federal University of Tocantins (UFT)
Postgraduate Program in Biosciences Interdisciplinary Center for Life Sciences and Nature Federal University of Latin American Integration (UNILA)
Bioprocess Engineering and Biotechnology Institute of Natural Resources Federal University of Itajubá
Department of Biochemistry and Chemical Technology Institute of Chemistry Universidade Estadual Paulista (UNESP)
Laboratory of Cell-Cell Interactions Morphology Department Institute of Biological Science Federal University of Minas Gerais (UFMG)
Department of Biochemistry and Chemical Technology Institute of Chemistry Universidade Estadual Paulista (UNESP)
