Autor(es): Prates, Janesly [UNESP] ; Moreli, Jusciéle Brogin ; Gimenes, Alexandre Dantas ; Biselli, Joice Matos [UNESP] ; Pires D'Avila, Solange Correa Garcia ; Sandri, Silvana ; Farsky, Sandra Helena Poliselli ; Rodrigues-Lisoni, Flávia Cristina [UNESP] ; Oliani, Sonia Maria [UNESP]
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/201139
Origem: Oasisbr
Assunto(s): Apoptosis; Cell proliferation; Gene expression; Peptide ANXA12-26; SiHa cells
Made available in DSpace on 2020-12-12T02:25:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Cisplatin (Cis) is a choice chemotherapy approach to cervical cancer by inducing DNA adducts and subsequent apoptosis. We have investigated the effects of Cis on Annexin A1 (ANXA1) and inhibitor of DNA binding 1 (ID1) proteins expression to elucidate further mechanisms of Cis actions. Human cervical tissue samples from twenty-four patients, with Cervical Intraepithelial Neoplasia (CIN, stage I, II and III), were evaluated to quantified ANXA1 and ID1 expressions. In vitro, human epidermoid carcinoma of the cervix (SiHa cell line) were treated with Annexin A1 peptide (ANXA12−26), Cis or Cis + ANXA12−26 to evaluate cell proliferation and migration, cytotoxicity of treatments as well as ANXA1 and ID1 modulations by mRNA and protein expression. Our findings showed expression of ID1 and ANXA1 proteins in tissue samples from Cervical Intraepithelial Neoplasia (CIN) patients, with intense immunological identification of ID1 in the CIN III stage. In SiHa cells, treatments with Cis alone or Cis + ANXA12−26, increase mRNA expressions of the ANXA1 and reduced the ID1. In agreement, Cis + ANXA12−26 enhanced ANXA1 protein expression and Cis or Cis + ANXA12−26 abolished ID1 protein expression. Cell proliferation was reduced after treatment with ANXA12−26 peptide and more significant after Cis or Cis + ANXA12−26 treatments. These two last treatments reduced cell viability, by inducing late apoptosis, and impaired cell migration. Together, our data highlight endogenous ANXA1 is involved in Cis therapy for cervical cancer.
São Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences (Ibilce), Campus São José do Rio Preto
Universidade Federal de São Paulo – UNIFESP Post-Graduation in Structural and Functional Biology
Faceres School of Medicine, São José do Rio Preto
Department of Pathology São José do Rio Preto School of Medicine (FAMERP), São José do Rio Preto
São Paulo University (USP) Department of Clinical and Toxicological Analysis Faculty of Pharmaceutical Sciences
São Paulo State University (Unesp) Ilha Solteira School of Engineering (FEIS), Campus Ilha Solteira
São Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences (Ibilce), Campus São José do Rio Preto
São Paulo State University (Unesp) Ilha Solteira School of Engineering (FEIS), Campus Ilha Solteira
CNPq: 140883/2014-2
FAPESP: 2016/02012-4
CNPq: 308144/2014-7
