Author(s):
Souza, Kátina Meneghetti [UNESP] ; De Vivo, Immaculata ; Chen, Chung-Yen ; Nogueira, Flávia Ribeiro [UNESP] ; Aun, Aline Garcia [UNESP] ; Arruda, Nayara Micarelli [UNESP] ; Lara, Juliana Rodrigues [UNESP] ; Silva, Mariane Aparecida P. [UNESP] ; Figueiredo, Drielle Baptista S. [UNESP] ; Corrêa, Camila Renata [UNESP] ; de Carvalho, Lídia Raquel [UNESP] ; Braz, José Reinaldo C. [UNESP] ; Braz, Leandro Gobbo [UNESP] ; Braz, Mariana Gobbo [UNESP]
Date: 2021
Persistent ID: http://hdl.handle.net/11449/205819
Origin: Oasisbr
Subject(s): indoor air pollution; inflammatory response; occupational exposure; oxidized DNA; transcriptional modulation; volatile anesthetics
Description
Made available in DSpace on 2021-06-25T10:21:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Considering the importance and lack of data of toxicogenomic approaches on occupational exposure to anesthetics, we evaluated possible associations between waste anesthetic gases (WAGs) exposure and biological effects including oxidative stress, DNA damage, inflammation, and transcriptional modulation. The exposed group was constituted by anesthesia providers who were mainly exposed to the anesthetics sevoflurane and isoflurane (10 ppm) and to a lesser degree to nitrous oxide (150 ppm), and the control group was constituted by physicians who had no exposure to WAGs. The oxidative stress markers included oxidized DNA bases (comet assay), malondialdehyde (high-performance liquid chromatography [HPLC]), nitric oxide metabolites (ozone-chemiluminescence), and antioxidative markers, including individual antioxidants (HPLC) and antioxidant defense marker (ferric reducing antioxidant power by spectrophotometry). The inflammatory markers included high-sensitivity C-reactive protein (chemiluminescent immunoassay) and the proinflammatory interleukins IL-6, IL-8 and IL-17A (flow cytometry). Telomere length and gene expression related to DNA repair (hOGG1 and XRCC1), antioxidant defense (NRF2) and inflammation (IL6, IL8 and IL17A) were evaluated by real-time quantitative polymerase chain reaction. No significant differences (p >.0025) between the groups were observed for any parameter evaluated. Thus, under the conditions of the study, the findings suggest that occupational exposure to WAGs is not associated with oxidative stress or inflammation when evaluated in serum/plasma, with DNA damage evaluated in lymphocytes and leucocytes or with molecular modulation assessed in peripheral blood cells in university anesthesia providers. However, it is prudent to reduce WAGs exposure and to increase biomonitoring of all occupationally exposed professionals.
Medical School São Paulo State University (UNESP)
Department of Epidemiology Harvard T.H. Chan School of Public Health
Channing Division of Network Medicine Department of Medicine Brigham and Women's Hospital and Harvard Medical School
Antioxidants Research Laboratory Jean Mayer Human Nutrition Research Center on Aging (HNRCA) Tufts University
UNIPEX Medical School São Paulo State University (UNESP)
Institute of Biosciences São Paulo State University (UNESP)
Medical School São Paulo State University (UNESP)
UNIPEX Medical School São Paulo State University (UNESP)
Institute of Biosciences São Paulo State University (UNESP)
CNPq: 147505/2018-6
CNPq: 149888/2019-8
FAPESP: 2016/155559-1
FAPESP: 2016/23902-8
FAPESP: 2017/18045-1
CNPq: 304107/2018-2
