Autor(es): Lopes, Leonardo G. ; Csonka, Larissa A. ; Castellane, Jessica A Souza ; Oliveira, Alef Winter ; de Almeida-Júnior, Sílvio ; Furtado, Ricardo Andrade ; Tararam, Cibele ; Levy, Larissa Ortolan ; Crivellenti, Leandro Zuccolotto ; Moretti, Maria Luiza ; Giannini, Maria José S Mendes [UNESP] ; Pires, Regina H.
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/206399
Origem: Oasisbr
Assunto(s): Aspergillus; biofilm; disinfectant; Fusarium; mice
Made available in DSpace on 2021-06-25T10:31:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
Aspergillus and Fusarium cause a broad spectrum of infections in humans, mainly in immunocompromised patients. Among these, patients undergoing hemodialysis are highly susceptible to infections, requiring a constant and adequate environmental disinfection program. Nevertheless, monitoring the residual disinfectants can contribute to the morbidity and mortality reduction in these patients. Here, we evaluated the susceptibility of Aspergillus spp. (n=19) and Fusarium spp. (n=13) environmental isolates against disinfectants (acetic acid, citric acid, peracetic acid, sodium hypochlorite, and sodium metabisulphite) at different concentrations and time exposures. Also, we investigated the in vivo toxicity of the peracetic acid residual concentration in mice. Fusarium isolates were identified by F. equiseti, F. oxysporum and F. solani while Aspergillus presented clinically relevant species (A. fumigatus, A. niger and A. terreus) and environmental ones. Against planktonic cells, only two disinfectants (acetic acid and sodium hypochlorite) showed a fungicidal effect on Fusarium spp., while only one (sodium hypochlorite) was effective against Aspergillus spp. Both fungi formed robust in vitro biofilms with large amounts of the extracellular matrix, as evidenced by electron micrographs. Exposure of fungal biofilms to disinfectants showed sensitivity to three (acetic, citric, and peracetic acids), although the concentrations and times of exposure varied according to the fungal genus. Mice exposure to the residual dose of peracetic acid during 60 weeks showed anatomopathological, hematological, and biochemical changes. The implementation of news control measures and those that already exist can help reduce infections, the second cause of death and morbidity in these patients, besides providing safety and well-being to them, a priority of any quality health program.
Postgraduate Program in Health Promotion University of Franca
Postgraduate Program in Animal Sciences University of Franca
Faculty of Medical Sciences University of Campinas
Faculty of Veterinary Medicine Federal University of Uberlandia
School of Pharmaceutical Sciences São Paulo State University (UNESP)
School of Pharmaceutical Sciences São Paulo State University (UNESP)
