Autor(es):
De Moura, Thales Reggiani [UNESP] ; Zanetti, Renan Diego [UNESP] ; Silva, Debora Eduarda Soares [UNESP] ; De Farias, Renan Lira [UNESP] ; Mauro, Antonio Eduardo [UNESP] ; Pereira, José Clayston Melo [UNESP] ; De Souza, Aline Aparecida ; Da Silva Siqueira, Fábio ; De Souza Júdice, Wagner Alves ; Lima, Mauro Almeida ; Rocha, Fillipe Vieira ; Deflon, Victor Marcelo ; De Godoy Netto, Adelino Vieira [UNESP]
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/206903
Origem: Oasisbr
Descrição
Made available in DSpace on 2021-06-25T10:45:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-07
Four palladium(ii) compounds of general formulae [PdCl(Ln)(PPh3)] {L1 = 3,5-dimethylpyrazole-1-iminothiolate (1); L2 = 3,5-dimethyl-pyrazole-N-methyl-1-iminothiolate (2); L3 = 3,5-dimethylpyrazole-N-ethyl-1-iminothiolate (3); L4 = 3,5-dimethylpyrazole-N-phenyl-1-iminothiolate (4); and PPh3 = triphenylphosphine} have been synthesized. The novel synthesized compounds have been characterized by C, H and N elemental analysis, 1D (1H and 13C) and 2D (HSQC and HMBC) NMR, MS, FT-IR, and molar electrical conductivity measurements. The molecular structure of complex 3 has been solved by single-crystal X-ray crystallography. The stability of the complexes in solution was studied in a DMSO/D2O (7 : 3) solution after 48 h. The antiproliferative activity of all free ligands and the stable palladium complexes 2-4 was assayed using the human breast tumour cell line MCF-7, lung tumour cell line A549 and human fetal lung fibroblast cell line MRC-5. Complex 3 was more active than cisplatin against MCF-7 cells, whilst palladium compounds 2-4 exhibited no drug response towards A549 cells at concentrations <50 μM. The binding properties of compounds 2 and 3 to ct-DNA have been studied using circular dichroism and fluorescence spectroscopy. The topoisomerase IIα inhibition has been studied for complex 2 and 3. The ability of all complexes to inhibit the activity of cathepsin B and L has also been investigated in this work. Compound 4 inhibited more than 50% of the cathepsin B activity at a concentration of 10 μM. Docking simulations have been carried out to gain more information about the interaction of the complexes and cathepsin B.
UNESP-Univ. Estadual Paulista Instituto de Química Departamento de Química Geral e Inorgânica
UMC - Univ. de Mogi das Cruzes Centro Interdisciplinar de Investigação Bioquímica
UFSCar-Univ. Federal de São Carlos Departamento de Química
USP-Univ. de São Paulo Instituto de Química de São Carlos
UNESP-Univ. Estadual Paulista Instituto de Química Departamento de Química Geral e Inorgânica
