Author(s): Gomes, Virgínia Juliani [UNESP] ; Rezeck Nunes, Priscila [UNESP] ; Haworth, Sarah McCann ; Sandrim, Valéria Cristina [UNESP] ; Peraçoli, José Carlos [UNESP] ; Peraçoli, Maria Terezinha S. [UNESP] ; Carlström, Mattias
Date: 2021
Persistent ID: http://hdl.handle.net/11449/207285
Origin: Oasisbr
Subject(s): HUVEC; monocytes; oxidative stress; preeclampsia; silibinin
Made available in DSpace on 2021-06-25T10:52:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
Objective: To investigate whether the supernatant from monocytes of preeclamptic and normotensive pregnant women, cultured in vitro with silibinin, can modulate oxidative stress in HUVEC. Methods: Concentrations of IL-1β, IL-10, and TNF-α in monocyte culture supernatants were determined by ELISA. HUVEC and their supernatant cultures were employed for determination of NO, nitrite and nitrate, lipid peroxidation, and hemeoxygenase-1 (HO-1). Results: HUVEC treatment with supernatant of preeclamptic monocytes cultured with silibinin produced increased levels of nitrite, reduced lipid peroxidation, and increased HO-1. Conclusion: Supernatant of monocytes from preeclamptic women induce oxidative stress in HUVEC which can be reduced by silibinin treatment. Abbreviations: DAF-FMTM, Diaminofluorescein-FM; EDTA, Ethylenediaminetetraacetic acid; HO-1, heme oxygenase-1; HPLC, high-performance liquid chromatography; HUVEC, human umbilical vein endothelial cell; MDA, malondialdehyde; NO, nitric oxide; NT, normotensive; PE, preeclampsia; ROS, reactive oxygen species; Sb, silibinin.
Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University (Unesp)
Department of Physiology and Pharmacology Karolinska Institutet
Department of Biological and Chemical Sciences Institute of Biosciences of Botucatu Sao Paulo State University (Unesp)
Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University (Unesp)
Department of Biological and Chemical Sciences Institute of Biosciences of Botucatu Sao Paulo State University (Unesp)
