Author(s): Mattos, Karine ; Cocio, Tiago Alexandre ; Chaves, Edilânia Gomes Araújo ; Borges, Clayton Luiz ; Venturini, James ; de Carvalho, Lídia Raquel [UNESP] ; Mendes, Rinaldo Poncio [UNESP] ; Paniago, Anamaria Mello Miranda ; Weber, Simone Schneider
Date: 2021
Persistent ID: http://hdl.handle.net/11449/207724
Origin: Oasisbr
Made available in DSpace on 2021-06-25T10:59:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01
Universidade de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul
Background Paracoccidioidomycosis (PCM) is a systemic and endemic fungal infection in Latin American, mainly in Brazil. The majority of PCM cases occur in large areas in Brazil, comprising the South, Southeast and Midwest regions, with the latter demonstrating a higher incidence of the species Paracoccidioides lutzii. Methodology and main findings This study presents clinical, molecular and serological data of thirteen new PCM cases during 2016 to 2019 from the state of Mato Grosso do Sul, located in the Midwest region, Brazil. From these thirteen cases, sixteen clinical isolates were obtained and their genomic DNAs were subjected to genotyping by tub1-PCR-RFLP. Results showed Paracoccidioides brasiliensis sensu stricto (S1) (11/16; 68.8%), Paracoccidioides restrepiensis (PS3) (4/16; 25.0%) and P. lutzii (1/16; 6.2%) as Paracoccidiodes species. Therefore, in order to understand whether the type of phylogenetic species that are circulating in the state influence the reactivity profile of serological tests, we performed double agar gel immunodiffusion (DID), using exoantigens from genotyped strains found in this series of PCM cases. Overall, our DID tests have been false negative in about 30% of confirmed PCM cases. All patients were male, most with current or previous rural activity, with ages ranging from 17 to 59 years, with 11 patients (84.6%) over 40 years of age. No clinical or epidemiological differences were found between Paracoccidioides species. However, it is important to note that the only case of P. lutzii died as an outcome. Conclusions This study suggests P. brasiliensis sensu stricto (S1) as the predominant species, showing its wide geographic distribution in Brazil. Furthermore, our findings revealed, for the first time, the occurrence of P. restrepiensis (PS3) in the state of Mato Grosso do Sul, Brazil. Despite our setbacks, it would be interesting to provide the complete sequencing of these clinical isolates to complement the molecular information presented.
Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias Faculdade de Medicina Universidade Federal de Mato Grosso do Sul
Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto (FMRP/USP) Universidade de São Paulo
Laboratório de Biologia Molecular Instituto de Ciências Biológicas Universidade Federal de Goiás
Universidade Estadual Paulista (UNESP) Departamento de Bioestatística do Instituto de Biociência de Botucatu
Universidade Estadual Paulista ‘Júlio Mesquita Filho’ (UNESP) Faculdade de Medicina de Botucatu
Laboratório de Biociência (LaBio) Faculdade de Ciências Farmacêuticas Alimentos e Nutrição Universidade Federal de Mato Grosso do Sul
Instituto de Ciências Exatas e Tecnologia (ICET) Universidade Federal do Amazonas
Universidade Estadual Paulista (UNESP) Departamento de Bioestatística do Instituto de Biociência de Botucatu
Universidade Estadual Paulista ‘Júlio Mesquita Filho’ (UNESP) Faculdade de Medicina de Botucatu
CAPES: 001
CNPq: 03/2016-PPSUS-MS
Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: 03/2016-PPSUS-MS
CAPES: 16/2014)
CNPq: 16/2014)
CNPq: PPSUS/FUNDECT No.08/2020
Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul: PPSUS/FUNDECT No.08/2020
