Author(s):
Pieri, Naira Caroline Godoy ; de Souza, Aline Fernanda ; Botigelli, Ramon Cesar [UNESP] ; Pessôa, Laís Vicari de Figueiredo ; Recchia, Kaiana ; Machado, Lucas Simões ; Glória, Mayra Hirakawa ; de Castro, Raquel Vasconcelos Guimarães [UNESP] ; Leal, Diego Feitosa ; Fantinato Neto, Paulo ; Martins, Simone Maria Massami Kitamura ; dos Santos Martins, Daniele ; Bressan, Fabiana Fernandes ; de Andrade, André Furugen Cesar
Date: 2021
Persistent ID: http://hdl.handle.net/11449/207956
Origin: Oasisbr
Subject(s): PGCs; Porcine; Regenerative medicine; Reproduction; Translational medicine
Description
Made available in DSpace on 2021-06-25T11:03:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells. Graphical abstract: [Figure not available: see fulltext.]
Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo
Department of Veterinary Medicine School of Animal Sciences and Food Engineering
Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP)
Department of Surgery Faculty of Veterinary Medicine and Animal Sciences University of Sao Paulo
Department of Biochemistry Paulista School of Medicine Federal University of São Paulo (UNIFESP)
Department of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
Department of Animal Science Faculty of Animal Science and Food Engineering University of São Paulo
Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP)
Department of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
