Author(s):
Andrade, Géssica ; Orlando, Haniel Chadwick Silva ; Scorzoni, Liliana [UNESP] ; Pedroso, Reginaldo Santos ; Abrão, Fariza ; Carvalho, Marco Túlio Menezes ; Veneziani, Rodrigo Cassio Sola ; Ambrósio, Sérgio Ricardo ; Bastos, Jairo Kenupp ; Mendes-Giannini, Maria José Soares [UNESP] ; Martins, Carlos Henrique Gomes ; Pires, Regina Helena
Date: 2021
Persistent ID: http://hdl.handle.net/11449/208067
Origin: Oasisbr
Subject(s): Antifungal; C. elegans–Candida infected model; Candida; Copaifera
Description
Made available in DSpace on 2021-06-25T11:05:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-01
Plants belonging to the genus Copaifera are widely used in Brazil due to their antimicrobial properties, among others. The re-emergence of classic fungal diseases as a consequence of antifungal resistance to available drugs has stimulated the search for plant-based compounds with antifungal activity, especially against Candida. The Candida-infected Caenorhabditis elegans model was used to evaluate the in vitro antifungal potential of Copaifera leaf extracts and trunk oleoresins against Candida species. The Copaifera leaf extracts exhibited good antifungal activity against all Candida species, with MIC values ranging from 5.86 to 93.75 µg/mL. Both the Copaifera paupera and Copaifera reticulata leaf extracts at 46.87 µg/mL inhibited Candida glabrata biofilm formation and showed no toxicity to C. elegans. The survival of C. glabrata-infected nematodes increased at all the tested extract concentrations. Exposure to Copaifera leaf extracts markedly increased C. glabrata cell vacuolization and cell membrane damage. Therefore, Copaifera leaf extracts are potential candidates for the development of new and safe antifungal agents.
University of Franca
School of Pharmaceutical Sciences São Paulo State University (UNESP)
Science and Technology Institute of São José dos Campos ICT São Paulo State University (UNESP)
Health Technical School (ESTES) Federal University of Uberlandia
Faculty of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo
Institute of Biomedical Sciences (ICBIM) Federal University of Uberlandia
School of Pharmaceutical Sciences São Paulo State University (UNESP)
Science and Technology Institute of São José dos Campos ICT São Paulo State University (UNESP)
