Author(s):
Conte, Fernanda Lopes [UNESP] ; Santiago, Karina Basso [UNESP] ; Conti, Bruno José [UNESP] ; Cardoso, Eliza de Oliveira [UNESP] ; Oliveira, Lucas Pires Garcia [UNESP] ; Feltran, Geórgia da Silva [UNESP] ; Zambuzzi, Willian Fernando [UNESP] ; Golim, Marjorie de Assis [UNESP] ; Cruz, Maria Teresa ; Sforcin, José Maurício [UNESP]
Date: 2021
Persistent ID: http://hdl.handle.net/11449/208571
Origin: Oasisbr
Subject(s): human monocytes; immunomodulation; inflammation; innate immunity; propolis
Description
Made available in DSpace on 2021-06-25T11:14:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-04
OBJECTIVES: Propolis is a bee-made product used for centuries due to its diverse biological properties, including its immunomodulatory action. This work aimed at investigating whether propolis may affect monocyte functions challenged with retinoic acid (RA), B subunit of Escherichia coli heat-labile enterotoxin (EtxB), human melanoma-associated antigen-1 (MAGE-1) and lipopolysaccharide (LPS). METHODS: Monocytes from healthy donors were treated with the stimuli separately or in the presence of propolis. Cell viability was evaluated by MTT assay, cell marker expression was assessed by flow cytometry, cytokine production by ELISA, gene expression by RT-qPCR. KEY FINDINGS: Propolis alone maintained TLR-2, TLR-4, HLA-DR, CD40 and CD80 expression in the monocytes; however, its combination with either MAGE-1 or LPS decreased CD40 expression triggered by the stimuli. Propolis maintained RA action on cell marker expression. Propolis inhibited TNF-α (with either EtxB or MAGE-1) and IL-6 (with either RA or MAGE-1), and increased IL-10 (with MAGE-1) production. Propolis downmodulated LC3 expression induced by LPS. It also induced a lower NF-kB expression than control cells and its combination with RA induced a higher expression than the stimulus alone. CONCLUSIONS: Propolis potentially affected innate immunity by downmodulating the monocytes pro-inflammatory activity.
Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
Botucatu Blood Center School of Medicine São Paulo State University (UNESP)
Center for Neurosciences and Cellular Biology Faculty of Pharmacy University of Coimbra
Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)
Botucatu Blood Center School of Medicine São Paulo State University (UNESP)
