Autor(es):
Pardo-Mora, Dolly Patricia ; Murillo, Oscar Julián ; Rey-Buitrago, Mauricio ; Losada-Barragán, Mónica ; Uribe, Jaime Fabian Cruz ; Santiago, Karina Basso [UNESP] ; Conti, Bruno José [UNESP] ; de Oliveira Cardoso, Eliza [UNESP] ; Conte, Fernanda Lopes [UNESP] ; Gutiérrez, Rafael María ; García, Orlando Torres ; Sforcin, José Maurício [UNESP]
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/208646
Origem: Oasisbr
Assunto(s): Apoptosis; Cytotoxicity; Osteosarcoma; Propolis
Descrição
Made available in DSpace on 2021-06-25T11:15:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01
Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.
Department of Animal Health Facultad de Medicina Veterinaria Universidad Antonio Nariño
Department of Morphology Facultad de Medicina Universidad Nacional de Colombia
Department of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of Biosciences
Department of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of Biosciences
