Autor(es): Figueira, Lucas Machado ; Alves, Nadja Gomes ; Rosa e Silva Maia, Ana Lucia ; Goncalves de Souza-Fabjan, Joanna Maria ; Tavares Pereira Batista, Ribrio Ivan ; Cruz Morais, Maria Clara da ; Lima, Renato Ribeiro de ; Franco Oliveira, Maria Emilia [UNESP] ; Fonseca, Jeferson Ferreira da
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/208793
Origem: Oasisbr
Assunto(s): Dairy sheep; MOET; Superovulation; Transcervical; Uterine flushing
Made available in DSpace on 2021-06-25T11:20:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01
Embrapa Goats and Sheep
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
This study assessed the effect of progestogen treatment length on ovarian parameters and embryo yield in superovulated Lacaune ewes collected by nonsurgical embryo recovery. Twenty-three lactating ewes were superovulated 30 d apart using a cross-over design. All ewes received 60 mg of MAP intravaginal sponges for 6 (G-6 group) or 9 (G-9 group) d. A total dose of 133 mg pFSH was given in six decreasing doses (twice a day) starting at 60 h before device removal. Ultrasound examination of the ovaries was performed at the first pFSH injection and one day before embryo recovery, which was performed 6-7 d after the onset of estrus. Embryo recovery was conducted only in ewes that expressed estrus and were mated. There was no difference (P>0.05) in the total number of follicles between G-6 (15.7 +/- 1.0) and G9 (15.6 +/- 0.8) at the time of the first pFSH treatment. The percentage of responding donors with >= 3 corpora lutea (CL; 78.2% [18/23] vs 69.5% [16/23]), mean (+/- SEM) CL number (7.0 +/- 1.2 vs 8.1 +/- 1.6), transcervical passage rate (94.4% [17/18] vs 83.3% [15/18], and ova/embryo recovery rate (54.5% [60/110] vs 68.0% [83/122]) were not different (P > 0.05) between the G-6 and G-9 groups. However, the mean number of viable embryos was lower (P < 0.05) in the G-6 group (1.8 +/- 0.7) than in the G-9 group. (3.5 +/- 1.1). In conclusion, treatment with an intravaginal MAP sponge for 9 d during a superovulation protocol is beneficial for viable embryo yield in Lacaune ewes out of the breeding season. (C) 2020 Elsevier Inc. All rights reserved.
Univ Fed Lavras, Av Doutor Sylvio Menicucci 1001, BR-37200900 Lavras, MG, Brazil
Univ Fed Fluminense, Rua Vital Brazil Filho 64, BR-24220000 Niteroi, RJ, Brazil
Univ Estadual Paulista, Via Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
Embrapa Caprinos & Ovinos, Estr Sobral Groairas,Km 4,CP D10, BR-62011000 Sobral, CE, Brazil
Univ Estadual Paulista, Via Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
Embrapa Goats and Sheep: Superov 22.13.06.026.00.03
Embrapa Goats and Sheep: 22.13.06.026.00.04
FAPEMIG: CVZ-PPM 00201-17
CAPES: 001
