Author(s): Souza, Thais T. S. ; Bezerra, Maria J. B. ; van Tilburg, Mauricio F. ; Nagano, Celso S. ; Rola, Luciana D. [UNESP] ; Duarte, Jose M. B. [UNESP] ; Melo, Luciana M. ; Moura, Arlindo A. ; Freitas, Vicente J. F.
Date: 2021
Persistent ID: http://hdl.handle.net/11449/208868
Origin: Oasisbr
Subject(s): Deer; Electrophoresis; MS; SDS-PAGE
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
(FUNCAP)
The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (<= 3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P> 0.05) was observed between small/medium and large follicles: 49.2 +/- 22.8 and 56.7 +/- 27.4 mu g/mu l, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P> 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.
Univ Estadual Ceara, Fac Vet, Av Dr Silas Munguba 1700, BR-60714903 Fortaleza, Ceara, Brazil
Univ Fed Ceara, Dept Anim Sci, Fortaleza, Ceara, Brazil
Univ Estadual Ceara, Hlth Sci Ctr, Fortaleza, Ceara, Brazil
Univ Fed Ceara, Lab Mass Spectrometry, Fortaleza, Ceara, Brazil
State Univ Sao Paulo, Dept Anim Sci, Jaboticabal, Brazil
Univ Ctr Fametro, Mol Genet Res Unit, Fortaleza, Ceara, Brazil
State Univ Sao Paulo, Dept Anim Sci, Jaboticabal, Brazil
CNPq: 461330/2014-8
(FUNCAP): CI3-0093-0005580100/14
