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In vivo embryo production and recovery in lacaune ewes after imposing a superovulation treatment regimen is related to pFSH dose


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Embrapa Goats and Sheep

Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

This study was conducted to assess effects of different doses of pFSH on follicular recruitment, superovulatory response, ova/embryo recovery, and embryo yield in lactating ewes. Ewes (n = 24) had a superovulation treatment regimen imposed. All ewes were implanted with a progesterone intravaginal device for 9 d, and administered either 100 (G-100) or 200 (G-200) mg pFSH, proportioned into six doses administered at 12-h intervals, starting 60 h before device removal. At 7 days subsequent to progesterone device removal, there were non-surgical embryo recoveries (NSER) from ewes having three or more corpora lutea. At the time of the first pFSH injection, number of antral follicles were similar (P < 0.05) between ewes in the G-100 and G-200 group, however, there were more 3.1-4.0 mm follicles in ewes of the G-200 than G-100 group at the time of the second pFSH administration. Estrous response and CL number were less (P < 0.05) in ewes of the G-100 (66.7 % and 2.6 +/- 0.7) than G-200 (91.7 % and 11.6 +/- 1.2) group. There were embryo collections from 100 % and 90.9 % of ewes in the G-100 and G-200 groups, respectively (P > 0.05). Viable embryo numbers and ova/embryo recovery rate were greater (P < 0.05) in ewes of the G-200 (6.9 +/- 1.1 and 67.8 %) than G-100 (1.0 +/- 0.5 and 27.6 %) group. A dose of 200 mg pFSH was more effective in inducing a superovulatory response and embryo yield after NSER in ewes, however, the 100 mg dose was insufficient for these purposes.

Univ Fed Lavras, Av Doutor Sylvio Menicucci 1001, BR-37200900 Lavras, MG, Brazil

Univ Fed Fluminense, Rua Vital Brazil Filho 6, BR-24230340 Niteroi, RJ, Brazil

Univ Fed Rural Rio de Janeiro, Rodovia BR 465,Km 07 S-N, BR-23890000 Seropedica, RJ, Brazil

Univ Estadual Paulista, Via Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, Brazil

Embrapa Caprinos & Ovinos, Estr Sobral Groairas,Km 4,CP D10, BR-62011000 Sobral, CE, Brazil

Univ Estadual Paulista, Via Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, Brazil

Embrapa Goats and Sheep: Superov 22.13.06.026.00.03/22.13.06.026.00.04

FAPEMIG: CVZ-PPM 00201-17

CAPES: 001

Tipo de Documento Artigo científico
Idioma Inglês
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