Author(s): Galetti, Renata ; Casarin Penha Filho, Rafael Antonio [UNESP] ; Ferreira, Joseane Cristina ; Varani, Alessandro M. [UNESP] ; Sazinas, Pavelas ; Jelsbak, Lars ; Costa Darini, Ana Lucia
Date: 2021
Persistent ID: http://hdl.handle.net/11449/209302
Origin: Oasisbr
Subject(s): Plasmids; Antibiotic resistance; Heavy metal tolerance; Poultry
Made available in DSpace on 2021-06-25T11:55:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Danish Council for Independent Research
The rapid emergence of resistant bacteria is occurring worldwide. The understanding of the dissemination of antimicrobial resistance using high-throughput sequencing and bioinformatics approaches is providing valuable insights into the genetic basis of the horizontal gene transfer and the emergence of the antibiotic resistance threat. This ultimately can offer vital clues to the development of coordinated efforts to implement new policies to continue fighting against bacterial infections. The poultry microbiota is characterized as a potential reservoir of resistance genes, mostly derived from the Enterobacteriaceae which have become increasingly important in human and animal infections. In this work, complete genome sequences were achieved for four multidrugresistant Salmonella spp. isolated from poultry from different farms in Brazil. We identified highly similar IncHI2-ST2 megaplasmids (larger than 275.000 bp) in all Salmonella isolates studied. These megaplasmids carry a resistome comprised of eleven different resistance genes (aac(6?)-Iaa, aadA1b, aph(4)-Ia, aph(6)-Id, aph(3?)-Ib, aph(3?)-Ia, aac(3)-Iva, sul1, tetA, tetB and dfrA1b) and four heavy metal tolerance operons (telluride, mercury, silver and copper). In conclusion, the multidrug-resistant plasmids identified in S. enterica serovar Schwarzengrund and Newport isolated from poultry show a variety of antibiotic resistance and heavy metal tolerance genes, providing advantages for the bacteria to survive under extremely unfavorable conditions.
Univ Sao Paulo, Fac Ciencias Fammceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, Brazil
Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
Tech Univ Denmark, Lyngby, Denmark
Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, Brazil
FAPESP: 2014/14494-8
CAPES: 001
FAPESP: 2015/11728-0
FAPESP: 2017/26311-3
Danish Council for Independent Research: 6108-00300A
