Author(s): Alves Silva, Evelin Carine [UNESP] ; Tanomaru-Filho, Mario [UNESP] ; Silva, Guilherme Ferreira da ; Delfino, Mateus Machado [UNESP] ; Cerri, Paulo Sergio [UNESP] ; Guerreiro-Tanomaru, Juliane Maria [UNESP]
Date: 2021
Persistent ID: http://hdl.handle.net/11449/209548
Origin: Oasisbr
Subject(s): Biocompatibility; calcium silicate; endodontics; root canal filling material
Made available in DSpace on 2021-06-25T12:21:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Introduction: Bio-C Sealer (BC; Angelus, Londrina, PR, Brazil) and Sealer Plus BC (SPBC; MK Life, Porto Alegre, Brazil) are new ready-to-use bioceramic endodontic sealers. The aim of this study was to evaluate the biocompatibility and bioactive potential of BC and SPBC sealers in comparison with AH Plus (AHP; Dentsply DeTrey Gmbh, Konstanz, Germany) in subcutaneous tissue of rats. Methods: Polyethylene tubes filled with materials and empty tubes, serving as the control group, were implanted in the subcutaneous tissues of rats. After 7, 15, 30, and 60 days, the tubes with connective tissue were removed, and inflammatory cells (ICs)/mm(2) and immunolabeled cells for interleukin (IL)-6 were evaluated. Osteocalcin and von Kossa analysis were also performed. Data were submitted to analysis of variance and Tukey tests, with a significance level of 5%. Results: At 7 days, SPBC showed lower ICs than BC (P < .05). AHP exhibited greater immunolabeled cells for IL-6 (P < .05). After 15 days, BC showed lower ICs and IL-6 compared with other materials. At 30 days, SPBC and AHP showed higher values for ICs (P < .05). After 60 days, calcium silicate sealers did not show statistical difference (P > .05) for ICs and IL-6, with values lower than AHP (P < .05). The materials showed positive structures to von Kossa staining. BC exhibited osteocalcin labeling in all periods. SPBC showed osteocalcin labeling from 15-60 days. AHP and the control group did not exhibit osteocalcin labeling. Conclusions: BC and SPBC sealers are biocompatible and present bioactive potential.
Sao Paulo State Univ, Sch Dent, Dept Restorat Dent, Rua Humaita 1680,PO 331, BR-14801903 Araraquara, SP, Brazil
Sao Paulo State Univ, Sch Dent, Dept Morphol, Araraquara, SP, Brazil
Ctr Univ Sagrad Coracao, Dept Dent, UNISAGRAD, Bauru, SP, Brazil
Sao Paulo State Univ, Sch Dent, Dept Restorat Dent, Rua Humaita 1680,PO 331, BR-14801903 Araraquara, SP, Brazil
Sao Paulo State Univ, Sch Dent, Dept Morphol, Araraquara, SP, Brazil
CAPES: 001
FAPESP: 2017/14305-9
FAPESP: 2017/19049-0
