Autor(es): Balderrama, Isis de Fatima [UNESP] ; Cardoso, Matheus Volz ; Stuani, Vitor Toledo ; Oliveira, Rodrigo Cardoso ; Matos, Adriana Arruda ; Aguiar Greghi, Sebastiao Luiz ; Passanezi Sant'Ana, Adriana Campos
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/209781
Origem: Oasisbr
Assunto(s): Dental implants; Decontamination; Osteoblast-like cells; Implant surface; Peri-implantitis
Made available in DSpace on 2021-06-25T12:29:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-17
Bauru School of Dentistry, University of Sao Paulo-USP
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Purpose To investigate the influence of implant surface decontaminated and uncontaminated on osteoblast-like cell adhesion and proliferation Materials and methods Commercially available implants of different brands and surface characteristics were selected: Biomet 3i (R) Nanotite (NT) and Osseotite (OT), Straumann (R) SLActive (SLA), and Neodent (R) Acqua Drive (ACQ) and Neoporos Drive CM (CM). Physical and chemical properties of the implants were investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and wettability analysis (WETT). Implants were previously contaminated with Aggregatibacter actinomycetemcomitans strains; after that, samples were decontaminated by different chemical methods. Decontaminated (test group; n = 15/type of implant) and uncontaminated (control group; n = 5/type of implant) samples were analyzed according to the number of human osteoblastic osteosarcoma cells (Saos-2) adhered on the implant surface after 24 h and 72 h in SEM images. Results ACQ was found to be highly hydrophilic, and NT was the most hydrophobic implant. Increased variation of Saos-2 cell adhesion and proliferation were observed on all test and control groups. Controversially, at the proliferation analysis in 72 h, CM implant was the only implant that showed no significant difference between test and group (p = 0.2833; Tukey's multiple comparisons test). NT implants showed the greater value of cell proliferation when compared with all types of implant surface (p = 0.0002; Tukey's multiple comparisons test). Conclusions These findings suggest that decontaminated surfaces were able to impair the counting of osteoblast-like cell adhesion and proliferation.
Sao Paulo State Univ, Araraquara Sch Dent, Dept Diag & Surg, Araraquara, SP, Brazil
Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Bauru, SP, Brazil
Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP, Brazil
Sao Paulo State Univ, Araraquara Sch Dent, Dept Diag & Surg, Araraquara, SP, Brazil
