Author(s): Thome, Maria Tereza C. [UNESP] ; Carstens, Bryan C. ; Rodrigues, Miguel T. ; Alexandrino, Joao ; Haddad, Celio F. B. [UNESP]
Date: 2021
Persistent ID: http://hdl.handle.net/11449/210051
Origin: Oasisbr
Subject(s): Caatinga; Cerrado; historical demography; Leptodactylus troglodytes; Neotropical diversification; Sao Francisco River
Made available in DSpace on 2021-06-25T12:38:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-19
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao Grupo Boticario de Protecao a Natureza
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Aim: Knowledge about the Neotropical dry formations, particularly the Caatinga, remains rudimentary compared to other biotas in the region. Here we address several biogeographical hypotheses by combining intense geographical and genomic sampling obtained for the Brazilian sibilator frog. We specifically test predictions related to the putative roles of past climate shifts (Pleistocene and Holocene) and local geographical barriers (past and current courses of the Sao Francisco River, SFR) in causing population differentiation in this species. Location: Caatinga and eastern Cerrado. Taxon: Brazilian sibilator frog Leptodactylus troglodytes. Methods: We sequenced up to similar to 15,000 single nucleotide polymorphisms for 159 samples from 61 locations. We inferred genetic structure using spatial clustering and examined population sizes through time. We estimated the relationship among populations using phylogenetic reconstruction, estimated historical distributions with ecological niche modelling and inferred demographic history under isolation-with-migration models. Results: Genetic diversity in L. troglodytes corresponds to biome boundaries, with one population in the Cerrado and two populations in the Caatinga, separated by the SFR. Demographic model selection indicates the Caatinga populations expanded since the end of the Pleistocene while the Cerrado population declined. Within the Caatinga, population expansion began earlier and was more extreme in the north. A continuous stability area maintained the two Caatinga populations, which share a common origin and began diverging in the mid-Pleistocene, first with symmetric gene flow and later under pronounced migration from the north. Main conclusions: We could not reject a role of past climate change in causing diversification of relictual populations in the Cerrado, but we found no evidence of multiple refuges or long-term isolation within the Caatinga. Instead, diversification in this biome appears to be caused by the SFR acting as a 'soft barrier' that constrains migration over an extended period of time.
Univ Estadual Paulista, Inst Biociencias, Dept Biodiversidade, Campus Rio Claro,Av 24A 1515, BR-13506900 Rio Claro, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, Ctr Aquicultura, Campus Rio Claro,Av 24A 1515, BR-13506900 Rio Claro, SP, Brazil
Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA
Univ Sao Paulo, Inst Biociencias, Dept Zool, Sao Paulo, SP, Brazil
Univ Fed Sao Paulo, Dept Ecol & Biol Evolut, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, Dept Biodiversidade, Campus Rio Claro,Av 24A 1515, BR-13506900 Rio Claro, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, Ctr Aquicultura, Campus Rio Claro,Av 24A 1515, BR-13506900 Rio Claro, SP, Brazil
Fundacao Grupo Boticario de Protecao a Natureza: 0909_ 20112
FAPESP: 2012/50255-2
FAPESP: 2003/103358
FAPESP: 2011/50146-6
FAPESP: 2013/50741-7
FAPESP: 2018/03428-5
FAPESP: 2011/51392-0
FAPESP: 2013/09088-8
