Author(s): Torres, Lidiane de Souza [UNESP] ; Silva, Danilo Grünig Humberto da [UNESP] ; Belini Junior, Edis [UNESP] ; Almeida, Eduardo Alves de [UNESP] ; Lobo, Clarisse Lopes de Castro ; Cançado, Rodolfo Delfini ; Ruiz, Milton Artur ; Bonini-Domingos, Claudia Regina [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/21452
Origin: Oasisbr
Subject(s): Hemoglobinopathies; Oxidative stress; Sickle cell anemia; Hydroxyurea
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
OBJECTIVE: The oxidative stress in 20 sickle cell anemia patients taking hydroxyurea and 13 sickle cell anemia patients who did not take hydroxyurea was compared with a control group of 96 individuals without any hemoglobinopathy. METHODS: Oxidative stress was assessed by thiobarbituric acid reactive species production, the Trolox-equivalent antioxidant capacity and plasma glutathione levels. RESULTS: Thiobarbituric acid reactive species values were higher in patients without specific medication, followed by patients taking hydroxyurea and the Control Group (p < 0.0001). The antioxidant capacity was higher in patients taking hydroxyurea and lower in the Control Group (p = 0.0002 for Trolox-equivalent antioxidant capacity and p < 0.0292 for plasma glutathione). Thiobarbituric acid reactive species levels were correlated with higher hemoglobin S levels (r = 0.55; p = 0.0040) and lower hemoglobin F concentrations(r = -0.52; p = 0.0067). on the other hand, plasma glutathione levels were negatively correlated with hemoglobin S levels (r = -0.49; p = 0.0111) and positively associated with hemoglobin F values (r = 0.56; p = 0.0031). CONCLUSION: Sickle cell anemia patients have high oxidative stress and, conversely, increased antioxidant activity. The increase in hemoglobin F levels provided by hydroxyurea and its antioxidant action may explain the reduction in lipid peroxidation and increased antioxidant defenses in these individuals.
Universidade Estadual Paulista
Instituto de Hematologia Arthur de Siqueira Cavalcanti
Santa Casa de São Paulo Faculdade de Ciências Médicas
Universidade de São Paulo
Universidade Estadual Paulista
